Cell Death Antibodies 1

Anti-Chk2 Antibody (CAB2145)

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SKU:
CAB2145
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Death

Description

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Antibody Name:Anti-Chk2 Antibody
Antibody SKU:CAB2145
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human Chk2 (NP_009125.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human
Positive Samples:HT-29, HeLa, DU145, H460, Raji, MCF-7, K-562, Jurkat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human Chk2 (NP_009125.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MSRE SDVE AQQS HGSS ACSQ PHGS VTQS QGSS SQSQ GISS SSTS TMPN SSQS SHSS SGTL SSLE TVST QELY SIPE DQEP EDQE PEEP TPAP WARL WALQ DGFA NLEC VNDN YWFG RDKS CEYC FDEP LLKR TDKY RTYS KKHF RIFR EVGP KNSY IAYI EDHS GNGT FVNT ELVG KGKR RPLN NNSE IALS LSRN KVFV FFDL TVDD QSVY PKAL RDEY
Gene ID:11200
Uniprot:O96017
Cellular Location:Nucleus, Nucleus, PML body, nucleoplasm
Calculated MW:15-38kDa/50-65kDa
Observed MW:70kDa
Synonyms:CDS1, CHK2, HuCds1, LFS2, PP1425, RAD53, hCds1, CHEK2, Chk2
Background:In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Several transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:Chk2: a CAMK protein kinase of the Rad53 family. A cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. Phosphorylates and inhibits CDC25C, preventing entry into mitosis, p53, leading to cell cycle arrest in G1, and BRCA1, restoring survival after DNA damage. Twelve splice variant isoforms have been described for human Chk2. LOF mutants cause Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype also caused by p53 mutations. Familial mutations also associated with prostate and breast cancer, and mutations also seen in a variety of sporadic cancers and cell lines.
UniProt Protein Details:

Protein type:EC 2.7.11.1; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, CAMK; Tumor suppressor; CAMK group; RAD53 family

Chromosomal Location of Human Ortholog: 22q12.1

Cellular Component: nucleoplasm; Golgi apparatus; PML body; chromosome, telomeric region

Molecular Function:protein serine/threonine kinase activity; identical protein binding; protein binding; protein homodimerization activity; metal ion binding; ubiquitin protein ligase binding; protein kinase binding; ATP binding

Biological Process: DNA damage induced protein phosphorylation; transcription, DNA-dependent; protein stabilization; protein amino acid autophosphorylation; positive regulation of transcription, DNA-dependent; DNA damage response, signal transduction; protein amino acid phosphorylation; regulation of transcription, DNA-dependent; DNA damage response, signal transduction resulting in induction of apoptosis; cell division; cellular protein catabolic process; double-strand break repair; DNA damage checkpoint; response to gamma radiation; regulation of protein catabolic process; G2/M transition of mitotic cell cycle; response to DNA damage stimulus

Disease: Li-fraumeni Syndrome 2; Breast Cancer; Prostate Cancer; Osteogenic Sarcoma

NCBI Summary:In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]
UniProt Code:O96017
NCBI GenInfo Identifier:6685284
NCBI Gene ID:11200
NCBI Accession:O96017.1
UniProt Secondary Accession:O96017,Q6QA03, Q6QA04, Q6QA05, Q6QA06, Q6QA07, Q6QA08 Q6QA10, A8K3Y9, B7ZBF3, B7ZBF4, B7ZBF5,
UniProt Related Accession:O96017
Molecular Weight:543
NCBI Full Name:Serine/threonine-protein kinase Chk2
NCBI Synonym Full Names:checkpoint kinase 2
NCBI Official Symbol:CHEK2  
NCBI Official Synonym Symbols:CDS1; CHK2; LFS2; RAD53; hCds1; HuCds1; PP1425  
NCBI Protein Information:serine/threonine-protein kinase Chk2; cds1 homolog; CHK2 checkpoint homolog; checkpoint-like protein CHK2
UniProt Protein Name:Serine/threonine-protein kinase Chk2
UniProt Synonym Protein Names:CHK2 checkpoint homolog; Cds1 homolog; Hucds1; hCds1; Checkpoint kinase 2
Protein Family:Serine/threonine-protein kinase
UniProt Gene Name:CHEK2  
UniProt Entry Name:CHK2_HUMAN
Anti-Chk2 Antibody (CAB2145)Western blot analysis of extracts of various cell lines, using Chk2 antibody (CAB2145) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Anti-Chk2 Antibody (CAB2145)Immunofluorescence analysis of MCF-7 cells using Chk2 antibody (CAB2145). Blue: DAPI for nuclear staining.
Anti-Chk2 Antibody (CAB2145)Immunofluorescence analysis of A549 cells using Chk2 antibody (CAB2145).
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