Cell Cycle Antibodies 1

Anti-CHFR Antibody (CAB10447)

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SKU:
CAB10447
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Cycle

Description

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Antibody Name:Anti-CHFR Antibody
Antibody SKU:CAB10447
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-290 of human CHFR (NP_001154818.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:HT-1080, 22Rv1, HepG2, Mouse kidney, Mouse testis
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-290 of human CHFR (NP_001154818.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MERP EEGK QSPP PQPW GRLL RLGA EEGE PHVL LRKR EWTI GRRR GCDL SFPS NKLV SGDH CRIV VDEK SGQV TLED TSTS GTVI NKLK VVKK QTCP LQTG DVIY LVYR KNEP EHNV AYLY ESLS EKQG MTQE SFDT SGAG AGRG ADPR VPPS SPAT QVCF EEPQ PSTS TSDL FPTA SASS TEPS PAGR ERSS SCGS GGGG ISPK GSGP SVAS DEVS SFAS ALPD RKTA SFSS LEPQ DQED LEPV KKKM RGDG DLDL NGQL LVAQ PRRN AQTV HEDV RAAA GKPD KMEE TL
Gene ID:55743
Uniprot:Q96EP1
Cellular Location:Nucleus, PML body
Calculated MW:63kDa/69kDa/72kDa/73kDa
Observed MW:70kDa
Synonyms:CHFR, RNF116, RNF196
Background:This gene encodes an E3 ubiquitin-protein ligase required for the maintenance of the antephase checkpoint that regulates cell cycle entry into mitosis and, therefore, may play a key role in cell cycle progression and tumorigenesis. The encoded protein has an N-terminal forkhead-associated domain, a central RING-finger domain, and a cysteine-rich C-terminal region. Alternatively spliced transcript variants that encode different protein isoforms have been described.
UniProt Protein Function:CHFR: E3 ubiquitin-protein ligase that functions in the antephase checkpoint by actively delaying passage into mitosis in response to microtubule poisons. Acts in early prophase before chromosome condensation, when the centrosome move apart from each other along the periphery of the nucleus. Probably involved in signaling the presence of mitotic stress caused by microtubule poisons by mediating the 'Lys-48'-linked ubiquitination of target proteins, leading to their degradation by the proteasome. Promotes the ubiquitination and subsequent degradation of AURKA and PLK1. Probably acts as a tumor suppressor, possibly by mediating the polyubiquitination of HDAC1, leading to its degradation. May also promote the formation of 'Lys-63'-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating UBC13-MMS2 (UBE2N-UBE2V2) heterodimer. Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation, but are rather involved in signaling cellular stress. Interacts with HDAC1 and HDAC2. Interacts with PML (with sumoylated form of PML). Ubiquitous. Belongs to the CHFR family. 5 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Ligase; EC 6.3.2.19; Ubiquitin conjugating system; EC 6.3.2.-; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 12q24.33

Cellular Component: PML body; nucleus

Molecular Function:protein binding; zinc ion binding; ubiquitin-protein ligase activity; nucleotide binding; ligase activity

Biological Process: ubiquitin-dependent protein catabolic process; modification-dependent protein catabolic process; mitosis; protein polyubiquitination; cell division; mitotic cell cycle checkpoint

NCBI Summary:This gene encodes an E3 ubiquitin-protein ligase required for the maintenance of the antephase checkpoint that regulates cell cycle entry into mitosis and, therefore, may play a key role in cell cycle progression and tumorigenesis. The encoded protein has an N-terminal forkhead-associated domain, a central RING-finger domain, and a cysteine-rich C-terminal region. Alternatively spliced transcript variants that encode different protein isoforms have been described. [provided by RefSeq, Mar 2014]
UniProt Code:Q96EP1
NCBI GenInfo Identifier:41688511
NCBI Gene ID:55743
NCBI Accession:Q96EP1.2
UniProt Secondary Accession:Q96EP1,Q96SL3, Q9NRT4, Q9NT32, Q9NVD5, A6NEN5, B4DZ77 B4E2L6,
UniProt Related Accession:Q96EP1
Molecular Weight:664
NCBI Full Name:E3 ubiquitin-protein ligase CHFR
NCBI Synonym Full Names:checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase
NCBI Official Symbol:CHFR  
NCBI Official Synonym Symbols:RNF116; RNF196  
NCBI Protein Information:E3 ubiquitin-protein ligase CHFR; RING finger protein 196
UniProt Protein Name:E3 ubiquitin-protein ligase CHFR
UniProt Synonym Protein Names:Checkpoint with forkhead and RING finger domains protein; RING finger protein 196
Protein Family:E3 ubiquitin-protein ligase
UniProt Gene Name:CHFR  
UniProt Entry Name:CHFR_HUMAN
Anti-CHFR Antibody (CAB10447)Western blot analysis of extracts of various cell lines, using CHFR antibody (CAB10447) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
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