Cell Biology Antibodies 3

Anti-CHCHD4 Antibody (CAB13139)

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SKU:
CAB13139
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-CHCHD4 Antibody
Antibody SKU:CAB13139
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-142 of human CHCHD4 (NP_001091972.1).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:100 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:LO2, 293T, Mouse liver, Mouse heart, Mouse pancreas
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-142 of human CHCHD4 (NP_001091972.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MSYC RQEG KDRI IFVT KEDH ETPS SAEL VADD PNDP YEEH GLIL PNGN INWN CPCL GGMA SGPC GEQF KSAF SCFH YSTE EIKG SDCV DQFR AMQE CMQK YPDL YPQE DEDE EEER EKKP AEQA EETA PIEA TATK EEEG SS
Gene ID:131474
Uniprot:Q8N4Q1
Cellular Location:Mitochondrion intermembrane space
Calculated MW:15kDa/17kDa
Observed MW:16kDa
Synonyms:CHCHD4, MIA40, TIMM40
Background:
UniProt Protein Function:CHCHD4: Required for the import and folding of small cysteine- containing proteins (small Tim) in the mitochondrial intermembrane space (IMS). Probably acts by forming a redox cycle with GFER/ERV1 that involves a disulfide relay system. Precursor proteins to be imported into the IMS are translocated in their reduced form into the mitochondria. The oxidized form of MIA40 forms a transient intermolecular disulfide bridge with the reduced precursor protein, resulting in oxidation of the precursor protein that now contains an intramolecular disulfide bond and is able to undergo folding in the IMS (Probable). 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Mitochondrial

Chromosomal Location of Human Ortholog: 3p25.1

Cellular Component: mitochondrial intermembrane space; mitochondrion

Molecular Function:protein disulfide oxidoreductase activity

Biological Process: 'de novo' posttranslational protein folding; protein import into mitochondrial intermembrane space; protein maturation via protein folding

NCBI Summary:CHCHD4, a component of human mitochondria, belongs to a protein family whose members share 6 highly conserved cysteine residues constituting a -CXC-CX(9)C-CX(9)C- motif in the C terminus (Hofmann et al., 2005 [PubMed 16185709]).[supplied by OMIM, Mar 2008]
UniProt Code:Q8N4Q1
NCBI GenInfo Identifier:62510498
NCBI Gene ID:131474
NCBI Accession:Q8N4Q1.1
UniProt Secondary Accession:Q8N4Q1,Q96AI2, Q96MY6, A8K3Z9,
UniProt Related Accession:Q8N4Q1
Molecular Weight:17,385 Da
NCBI Full Name:Mitochondrial intermembrane space import and assembly protein 40
NCBI Synonym Full Names:coiled-coil-helix-coiled-coil-helix domain containing 4
NCBI Official Symbol:CHCHD4  
NCBI Official Synonym Symbols:MIA40; TIMM40  
NCBI Protein Information:mitochondrial intermembrane space import and assembly protein 40
UniProt Protein Name:Mitochondrial intermembrane space import and assembly protein 40
UniProt Synonym Protein Names:Coiled-coil-helix-coiled-coil-helix domain-containing protein 4
Protein Family:Mitochondrial intermembrane space import and assembly protein
UniProt Gene Name:CHCHD4  
UniProt Entry Name:MIA40_HUMAN
Anti-CHCHD4 Antibody (CAB13139)Western blot analysis of extracts of various cell lines, using CHCHD4 antibody (CAB13139) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
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