Antibody Name:	Anti-CHCHD3 Antibody
Antibody SKU:	CAB8584
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-227 of human CHCHD3 (NP_060282.1).

Application:	WB IHC IF IP
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:200 IP 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	A-549, HeLa, A-431, Jurkat, HepG2, mouse kidney, mouse liver, rat heart, rat kidney, Mouse kidney, Mouse liver, Mouse heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-227 of human CHCHD3 (NP_060282.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MGGT TSTR RVTF EADE NENI TVVK GIRL SENV IDRM KESS PSGS KSQR YSGA YGAS VSDE ELKR RVAE ELAL EQAK KESE DQKR LKQA KELD RERA AANE QLTR AILR ERIC SEEE RAKA KHLA RQLE EKDR VLKK QDAF YKEQ LARL EERS SEFY RVTT EQYQ KAAE EVEA KFKR YESH PVCA DLQA KILQ CYRE NTHQ TLKC SALA TQYM HCVN HAKQ SMLE KGG
Gene ID:	54927
Uniprot:	Q9NX63
Cellular Location:	Cytoplasm, Intermembrane side, Lipid-anchor, Mitochondrion, Mitochondrion inner membrane, Nucleus
Calculated MW:	26kDa
Observed MW:	30kDa

Synonyms:

CHCHD3, MINOS3, Mic19, PPP1R22

Background:

The protein encoded by this gene is an inner mitochondrial membrane scaffold protein. Absence of the encoded protein affects the structural integrity of mitochondrial cristae and leads to reductions in ATP production, cell growth, and oxygen consumption. This protein is part of the mitochondrial contact site and cristae organizing system (MICOS). Several transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	CHCHD3: May be a scaffolding protein that stabilizes protein complexes involved in maintaining mitochondrial crista architecture and protein import.
UniProt Protein Details:	Protein type:Mitochondrial Chromosomal Location of Human Ortholog: 7q33 Cellular Component: cytoplasm; mitochondrial inner membrane; mitochondrion; nucleus Molecular Function:phosphatase binding; protein binding; protein complex scaffold Biological Process: cristae formation; inner mitochondrial membrane organization and biogenesis; mitochondrial fusion; negative regulation of transcription from RNA polymerase II promoter
NCBI Summary:	The protein encoded by this gene is an inner mitochondrial membrane scaffold protein. Absence of the encoded protein affects the structural integrity of mitochondrial cristae and leads to reductions in ATP production, cell growth, and oxygen consumption. This protein is part of the mitochondrial contact site and cristae organizing system (MICOS). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2015]
UniProt Code:	Q9NX63
NCBI GenInfo Identifier:	62510520
NCBI Gene ID:	54927
NCBI Accession:	Q9NX63.1
UniProt Related Accession:	Q9NX63
Molecular Weight:	26,152 Da
NCBI Full Name:	MICOS complex subunit MIC19
NCBI Synonym Full Names:	coiled-coil-helix-coiled-coil-helix domain containing 3
NCBI Official Symbol:	CHCHD3
NCBI Official Synonym Symbols:	Mic19; MINOS3; PPP1R22
NCBI Protein Information:	MICOS complex subunit MIC19
UniProt Protein Name:	MICOS complex subunit MIC19
UniProt Synonym Protein Names:	Coiled-coil-helix-coiled-coil-helix domain-containing protein 3
UniProt Gene Name:	CHCHD3
UniProt Entry Name:	MIC19_HUMAN

	Western blot analysis of extracts of various cell lines, using CHCHD3 antibody (CAB8584) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 40s.
	Immunohistochemistry of paraffin-embedded mouse brain using CHCHD3 antibody (CAB8584) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat brain using CHCHD3 antibody (CAB8584) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse lung using CHCHD3 antibody (CAB8584) at dilution of 1:100 (40x lens).
	Confocal immunofluorescence analysis of U2OS cells using Anti-CHCHD3 Polyclonal Antibody (CAB8584) at dilution of 1:100. Blue: DAPI for nuclear staining.