KO Validated Antibodies 2

Anti-CHCHD3 Antibody (CAB19959)[KO Validated]

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SKU:
CAB19959
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-CHCHD3 Antibody [KO Validated]
Antibody SKU:CAB19959
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant protein of human CHCHD3.
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:293T
Immunogen:Recombinant protein of human CHCHD3.
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:54927
Uniprot:Q9NX63
Cellular Location:Cytoplasm, Intermembrane side, Lipid-anchor, Mitochondrion, Mitochondrion inner membrane, Nucleus
Calculated MW:26kDa
Observed MW:26KDa
Anti-CHCHD3 Antibody [KO Validated] (CAB19959)Western blot analysis of extracts from normal (control) and CHCHD3 knockout (KO) 293T cells, using CHCHD3 antibody (CAB19959) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Synonyms:CHCHD3, MINOS3, Mic19, PPP1R22
Background:The protein encoded by this gene is an inner mitochondrial membrane scaffold protein. Absence of the encoded protein affects the structural integrity of mitochondrial cristae and leads to reductions in ATP production, cell growth, and oxygen consumption. This protein is part of the mitochondrial contact site and cristae organizing system (MICOS). Several transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:CHCHD3: May be a scaffolding protein that stabilizes protein complexes involved in maintaining mitochondrial crista architecture and protein import.
UniProt Protein Details:

Protein type:Mitochondrial

Chromosomal Location of Human Ortholog: 7q33

Cellular Component: cytoplasm; mitochondrial inner membrane; mitochondrion; nucleus

Molecular Function:phosphatase binding; protein binding; protein complex scaffold

Biological Process: cristae formation; inner mitochondrial membrane organization and biogenesis; mitochondrial fusion; negative regulation of transcription from RNA polymerase II promoter

NCBI Summary:The protein encoded by this gene is an inner mitochondrial membrane scaffold protein. Absence of the encoded protein affects the structural integrity of mitochondrial cristae and leads to reductions in ATP production, cell growth, and oxygen consumption. This protein is part of the mitochondrial contact site and cristae organizing system (MICOS). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2015]
UniProt Code:Q9NX63
NCBI GenInfo Identifier:62510520
NCBI Gene ID:54927
NCBI Accession:Q9NX63.1
UniProt Related Accession:Q9NX63
Molecular Weight:26,152 Da
NCBI Full Name:MICOS complex subunit MIC19
NCBI Synonym Full Names:coiled-coil-helix-coiled-coil-helix domain containing 3
NCBI Official Symbol:CHCHD3  
NCBI Official Synonym Symbols:Mic19; MINOS3; PPP1R22  
NCBI Protein Information:MICOS complex subunit MIC19
UniProt Protein Name:MICOS complex subunit MIC19
UniProt Synonym Protein Names:Coiled-coil-helix-coiled-coil-helix domain-containing protein 3
UniProt Gene Name:CHCHD3  
UniProt Entry Name:MIC19_HUMAN
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