Neuroscience

Anti-CHAT Antibody (CAB13244)

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SKU:
CAB13244
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Neuroscience

Description

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Antibody Name:Anti-CHAT Antibody
Antibody SKU:CAB13244
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 331-630 of human CHAT (NP_001136401.1).
Application:WB IHC IF
Recommended Dilution:WB 1:1000 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:U-87MG, HeLa, SP2/0
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 331-630 of human CHAT (NP_001136401.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:VLVQ CTEH LLKH MTQS SRKL IRAD SVSE LPAP RRLR WKCS PEIQ GHLA SSAE KLQR IVKN LDFI VYKF DNYG KTFI KKQK CSPD AFIQ VALQ LAFY RLHR RLVP TYES ASIR RFQE GRVD NIRS ATPE ALAF VRAV TDHK AAVP ASEK LLLL KDAI RAQT AYTV MAIT GMAI DNHL LALR ELAR AMCK ELPE MFMD ETYL MSNR FVLS TSQV PTTT EMFC CYGP VVPN GYGA CYNP QPET ILFC ISSF HSCK ETSS SKFA KAVE ESLI DMRD LCSL LPPT ESKP LATK EKAT RPSQ GHQP
Gene ID:1103
Uniprot:P28329
Cellular Location:
Calculated MW:70kDa/74kDa/82kDa
Observed MW:70kDa
Synonyms:CHAT, CHOACTASE, CMS1A, CMS1A2, CMS6
Background:This gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer's disease. Polymorphisms in this gene have been associated with Alzheimer's disease and mild cognitive impairment. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform.
UniProt Protein Function:CHAT: an enzyme that catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. Four alternative splice variants have been described.
UniProt Protein Details:

Protein type:EC 2.3.1.6; Lipid Metabolism - glycerophospholipid; Acetyltransferase

Chromosomal Location of Human Ortholog: 10q11.2

Cellular Component: cell soma; mitochondrion; axon; cytoplasm; nucleus; cytosol

Molecular Function:choline O-acetyltransferase activity

Biological Process: rhythmic excitation; muscle development; neurotransmitter secretion; glycerophospholipid biosynthetic process; adult walking behavior; synaptic transmission; rhythmic behavior; phospholipid metabolic process; phosphatidylcholine biosynthetic process; dendrite development; neuromuscular synaptic transmission; establishment of synaptic specificity at neuromuscular junction; neurotransmitter biosynthetic process

Disease: Myasthenic Syndrome, Congenital, Associated With Episodic Apnea

NCBI Summary:This gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer's disease. Polymorphisms in this gene have been associated with Alzheimer's disease and mild cognitive impairment. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform. [provided by RefSeq, May 2010]
UniProt Code:P28329
NCBI GenInfo Identifier:281185509
NCBI Gene ID:1103
NCBI Accession:P28329.4
UniProt Secondary Accession:P28329,Q16488, Q9BQ23, Q9BQ35, Q9BQE1, A2BDF4, A2BDF5
UniProt Related Accession:P28329
Molecular Weight:748
NCBI Full Name:Choline O-acetyltransferase
NCBI Synonym Full Names:choline O-acetyltransferase
NCBI Official Symbol:CHAT  
NCBI Official Synonym Symbols:CMS1A; CMS1A2; CHOACTASE  
NCBI Protein Information:choline O-acetyltransferase; choline acetylase; acetyl CoA:choline O-acetyltransferase
UniProt Protein Name:Choline O-acetyltransferase
Protein Family:(Z)-3-hexen-1-ol acetyltransferase
UniProt Gene Name:CHAT  
UniProt Entry Name:CLAT_HUMAN
Anti-CHAT Antibody (CAB13244)Western blot analysis of extracts of various cell lines, using CHAT antibody (CAB13244) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Anti-CHAT Antibody (CAB13244)Immunofluorescence analysis of NIH/3T3 cells using CHAT antibody (CAB13244) at dilution of 1:100. Blue: DAPI for nuclear staining.
Anti-CHAT Antibody (CAB13244)Immunofluorescence analysis of THP-1 cells using CHAT antibody (CAB13244) at dilution of 1:100. Blue: DAPI for nuclear staining.
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