Cell Cycle Antibodies 1

Anti-CHAF1A Antibody (CAB2244)

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SKU:
CAB2244
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Cycle

Description

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Antibody Name:Anti-CHAF1A Antibody
Antibody SKU:CAB2244
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human CHAF1A (NP_005474.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:HeLa, K-562
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human CHAF1A (NP_005474.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MLEE LECG APGA RGAA TAMD CKDR PAFP VKKL IQAR LPFK RLNL VPKG KADD MSDD QGTS VQSK SPDL EASL DTLE NNCH VGSD IDFR PKLV NGKG PLDN FLRN RIET SIGQ STVI IDLT EDSN EQPD SLVD HNKL NSEA SPSR EAIN GQRE DTGD QQGL LKAI QNDK LAFP GETL SDIP CKTE EEGV GCGG AGRR GDSQ
Gene ID:10036
Uniprot:Q13111
Cellular Location:Nucleus
Calculated MW:84kDa/88kDa/106kDa
Observed MW:160kDa
Synonyms:CHAF1A, CAF-1, CAF1, CAF1B, CAF1P150, P150
Background:
UniProt Protein Function:CAF-1A: Core component of the CAF-1 complex, a complex thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. CHAF1A binds to histones H3 and H4. It may play a role in heterochromatin maintenance in proliferating cells by bringing newly synthesized cbx proteins to heterochromatic DNA replication foci. The CCR4-NOT complex functions as general transcription regulation complex. Also involved in vitamin D- coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR- mediated transrepression of the CYP27B1 gene. Homodimer. Part of the CAF-1 complex that contains RBBP4, CHAF1B and CHAF1A. CHAF1A binds directly to CHAF1B. Only minor amounts of RBBP4 are complexed with CHAF1A and CHAF1B in G1 phase. Part of the CCR4-NOT core complex that contains CHAF1A, CHAF1B, CNOT1, CNOT2, CNOT3, CNOT4, CNOT6 and CNOT8. CHAF1A binds directly to PCNA and to CBX1. Binds MBD1. Interacts directly with CBX5 via the PxVxL motif. During DNA replication, it forms a S phase- specific complex that facilitates DNA methylation and histone H3 'Lys-9' methylation during replication-coupled chromatin assembly and is at least composed of the CHAF1A, MBD1 and SETDB1. Component of the WINAC complex, at least composed of SMARCA2, SMARCA4, SMARCB1, SMARCC1, SMARCC2, SMARCD1, SMARCE1, ACTL6A, BAZ1B/WSTF, ARID1A, SUPT16H, CHAF1A and TOP2B. Interacts with CBX5. Belongs to the CHAF1A family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:DNA replication

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: protein complex; nuclear chromatin

Molecular Function:identical protein binding; protein binding; unfolded protein binding; chromatin binding

Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent; chromatin assembly; protein complex assembly; DNA repair; cell cycle; DNA replication; DNA replication-dependent nucleosome assembly

NCBI Summary:Chromatin assembly factor I (CAF1) is a nuclear complex consisting of p50, p60 (CHAF1B; MIM 601245), and p150 (CHAF1A) subunits that assembles histone octamers onto replicating DNA in vitro (Kaufman et al., 1995 [PubMed 7600578]).[supplied by OMIM, Mar 2008]
UniProt Code:Q13111
NCBI GenInfo Identifier:229462842
NCBI Gene ID:10036
NCBI Accession:Q13111.2
UniProt Secondary Accession:Q13111,Q6NXG5, Q7Z7K3, Q9UJY8,
UniProt Related Accession:Q13111
Molecular Weight:956
NCBI Full Name:Chromatin assembly factor 1 subunit A
NCBI Synonym Full Names:chromatin assembly factor 1, subunit A (p150)
NCBI Official Symbol:CHAF1A  
NCBI Official Synonym Symbols:CAF1; P150; CAF-1; CAF1B; CAF1P150  
NCBI Protein Information:chromatin assembly factor 1 subunit A; hp150; CAF-I p150; CAF-1 subunit A; CAF-I 150 kDa subunit; chromatin assembly factor I (150 kDa); chromatin assembly factor I p150 subunit
UniProt Protein Name:Chromatin assembly factor 1 subunit A
UniProt Synonym Protein Names:Chromatin assembly factor I p150 subunit; CAF-I 150 kDa subunit; CAF-I p150; hp150
Protein Family:Chromatin assembly factor
UniProt Gene Name:CHAF1A  
UniProt Entry Name:CAF1A_HUMAN
Anti-CHAF1A Antibody (CAB2244)Western blot analysis of extracts of various cell lines, using CHAF1A antibody (CAB2244) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
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