Signal Transduction Antibodies 2
Anti-CHAD Antibody (CAB14985)
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- SKU:
- CAB14985
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Signal Transduction
Description
| Antibody Name: | Anti-CHAD Antibody |
| Antibody SKU: | CAB14985 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-359 of human CHAD (NP_001258.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human |
| Positive Samples: | U-251MG, MCF7 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-359 of human CHAD (NP_001258.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MVRP MLLL SLGL LAGL LPAL AACP QNCH CHSD LQHV ICDK VGLQ KIPK VSEK TKLL NLQR NNFP VLAA NSFR AMPN LVSL HLQH CQIR EVAA GAFR GLKQ LIYL YLSH NDIR VLRA GAFD DLTE LTYL YLDH NKVT ELPR GLLS PLVN LFIL QLNN NKIR ELRA GAFQ GAKD LRWL YLSE NALS SLQP GALD DVEN LAKF HVDR NQLS SYPS AALS KLRV VEEL KLSH NPLK SIPD NAFQ SFGR YLET LWLD NTNL EKFS DGAF LGVT TLKH VHLE NNRL NQLP SNFP FDSL ETLA LTNN PWKC TCQL RGLR RWLE AKAS RPDA TCAS PAKF KGQH IRDT DAFR SCKF PTKR SKKA GRH |
| Gene ID: | 1101 |
| Uniprot: | O15335 |
| Cellular Location: | Secreted, extracellular matrix, extracellular space |
| Calculated MW: | 40kDa |
| Observed MW: | 40kDa |
| Synonyms: | CHAD, SLRR4A |
| Background: | Chondroadherin is a cartilage matrix protein thought to mediate adhesion of isolated chondrocytes. The protein contains 11 leucine-rich repeats flanked by cysteine-rich regions. The chondroadherin messenger RNA is present in chondrocytes at all ages. |
| UniProt Protein Function: | Function: Promotes attachment of chondrocytes, fibroblasts, and osteoblasts. This binding is mediated (at least for chondrocytes and fibroblasts) by the integrin alpha2beta1. May play an important role in the regulation of chondrocyte growth and proliferation |
| NCBI Summary: | Chondroadherin is a cartilage matrix protein thought to mediate adhesion of isolated chondrocytes. The protein contains 11 leucine-rich repeats flanked by cysteine-rich regions. The chondroadherin messenger RNA is present in chondrocytes at all ages. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O15335 |
| NCBI GenInfo Identifier: | 153251229 |
| NCBI Gene ID: | 1101 |
| NCBI Accession: | NP_001258.2 |
| UniProt Secondary Accession: | O15335,Q6GTU0, Q96RJ5, A8K812, |
| UniProt Related Accession: | O15335 |
| Molecular Weight: | 40,476 Da |
| NCBI Full Name: | chondroadherin |
| NCBI Synonym Full Names: | chondroadherin |
| NCBI Official Symbol: | CHAD |
| NCBI Official Synonym Symbols: | SLRR4A |
| NCBI Protein Information: | chondroadherin; chondroadherin proteoglycan; cartilage leucine-rich protein |
| UniProt Protein Name: | Chondroadherin |
| UniProt Synonym Protein Names: | Cartilage leucine-rich protein |
| Protein Family: | Chondroadherin |
| UniProt Gene Name: | CHAD |
| UniProt Entry Name: | CHAD_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CHAD antibody (CAB14985) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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