Cell Biology Antibodies 7
Anti-CFL1 Antibody (CAB1704)
- SKU:
- CAB1704
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
Antibody Name: | Anti-CFL1 Antibody |
Antibody SKU: | CAB1704 |
Antibody Size: | 20uL, 50uL, 100uL |
Application: | WB IHC IF |
Reactivity: | Human, Mouse, Rat |
Host Species: | Rabbit |
Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human CFL1 (NP_005498.1). |
Application: | WB IHC IF |
Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
Reactivity: | Human, Mouse, Rat |
Positive Samples: | HeLa, Jurkat, SH-SY5Y, MCF7, Mouse brain, Mouse testis, Rat brain, Rat ovary |
Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human CFL1 (NP_005498.1). |
Purification Method: | Affinity purification |
Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Isotype: | IgG |
Sequence: | MASG VAVS DGVI KVFN DMKV RKSS TPEE VKKR KKAV LFCL SEDK KNII LEEG KEIL VGDV GQTV DDPY ATFV KMLP DKDC RYAL YDAT YETK ESKK EDLV |
Gene ID: | 1072 |
Uniprot: | P23528 |
Cellular Location: | Cell projection, Cytoplasm, Cytoplasmic side, Nucleus matrix, Peripheral membrane protein, cytoskeleton, lamellipodium membrane, ruffle membrane |
Calculated MW: | 18kDa |
Observed MW: | 18kDa |
Synonyms: | CFL1, CFL, HEL-S-15, cofilin, cofilin-1 |
Background: |
UniProt Protein Function: | Cofilin-1: a cytoskeletal protein that controls actin depolymerization. Has a 5-10-fold higher affinity for ATP-actin monomers than cofilin-1. May promote filament assembly rather than disassembly. Two alternatively spliced variants are described. Isoform b is expressed predominantly in skeletal muscle and heart, while isoform a is expressed in various tissues. |
UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Nuclear receptor co-regulator; Cytoskeletal Chromosomal Location of Human Ortholog: 11q13 Cellular Component: cortical actin cytoskeleton; cytoplasm; extracellular space; focal adhesion; intercellular junction; membrane; nuclear matrix; nucleus; vesicle Molecular Function:actin binding; protein binding Biological Process: actin cytoskeleton organization and biogenesis; actin filament depolymerization; axon guidance; blood coagulation; cytokinesis after mitosis; cytoskeleton organization and biogenesis; ephrin receptor signaling pathway; establishment of cell polarity; innate immune response; negative regulation of apoptosis; neural crest cell migration; neural fold formation; platelet activation; platelet degranulation; positive regulation of actin filament depolymerization; protein amino acid phosphorylation; regulation of cell morphogenesis; response to amino acid stimulus; response to virus; Rho protein signal transduction |
NCBI Summary: | The protein encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH-dependent manner. Increased phosphorylation of this protein by LIM kinase aids in Rho-induced reorganization of the actin cytoskeleton. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM, Apr 2004] |
UniProt Code: | P23528 |
NCBI GenInfo Identifier: | 116848 |
NCBI Gene ID: | 1072 |
NCBI Accession: | P23528.3 |
UniProt Secondary Accession: | P23528,Q53Y87, Q9UCA2, B3KUQ1, |
UniProt Related Accession: | P23528 |
Molecular Weight: | 18,502 Da |
NCBI Full Name: | Cofilin-1 |
NCBI Synonym Full Names: | cofilin 1 |
NCBI Official Symbol: | CFL1 |
NCBI Official Synonym Symbols: | CFL; cofilin; HEL-S-15 |
NCBI Protein Information: | cofilin-1 |
UniProt Protein Name: | Cofilin-1 |
UniProt Synonym Protein Names: | 18 kDa phosphoprotein; p18; Cofilin, non-muscle isoform |
Protein Family: | Cofilin |
UniProt Gene Name: | CFL1 |
UniProt Entry Name: | COF1_HUMAN |