Name: Anti-CETN2 Antibody (CAB5397)[KO Validated]
Brand: KO Validated Antibodies 1
SKU: CAB5397
Availability: OutOfStock

Antibody Name:	Anti-CETN2 Antibody [KO Validated]
Antibody SKU:	CAB5397
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-172 of human CETN2 (NP_004335.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, 293T, Mouse brain, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-172 of human CETN2 (NP_004335.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MASN FKKA NMAS SSQR KRMS PKPE LTEE QKQE IREA FDLF DADG TGTI DVKE LKVA MRAL GFEP KKEE IKKM ISEI DKEG TGKM NFGD FLTV MTQK MSEK DTKE EILK AFKL FDDD ETGK ISFK NLKR VAKE LGEN LTDE ELQE MIDE ADRD GDGE VSEQ EFLR IMKK TSLY
Gene ID:	1069
Uniprot:	P41208
Cellular Location:	Cytoplasm, Nucleus, centriole, centrosome, cytoskeleton, microtubule organizing center
Calculated MW:	19kDa
Observed MW:	20KDa

Synonyms:	CETN2, CALT, CEN2, centrin-2
Background:	Caltractin belongs to a family of calcium-binding proteins and is a structural component of the centrosome. The high level of conservation from algae to humans and its association with the centrosome suggested that caltractin plays a fundamental role in the structure and function of the microtubule-organizing center, possibly required for the proper duplication and segregation of the centrosome.

UniProt Protein Function:	CETN2: a calcium-binding proteins and is a structural component of the centrosome. Contains 4 EF-hand calcium-binding domains. Highly conserved from algae to humans. Its association with the centrosome suggests that it plays a fundamental role in the structure and function of the microtubule-organizing center, possibly required for the proper duplication and segregation of the centrosome.
UniProt Protein Details:	Protein type:DNA repair, damage; Calcium-binding; Cell cycle regulation Chromosomal Location of Human Ortholog: Xq28 Cellular Component: centriole; centrosome; intracellular; cytosol; photoreceptor connecting cilium Molecular Function:protein binding; microtubule binding; G-protein beta/gamma-subunit binding; calcium ion binding; heterotrimeric G-protein binding Biological Process: mitosis; cell division; nucleotide-excision repair; organelle organization and biogenesis; centriole replication; spermatogenesis; mitotic cell cycle; G2/M transition of mitotic cell cycle; regulation of cytokinesis
NCBI Summary:	Caltractin belongs to a family of calcium-binding proteins and is a structural component of the centrosome. The high level of conservation from algae to humans and its association with the centrosome suggested that caltractin plays a fundamental role in the structure and function of the microtubule-organizing center, possibly required for the proper duplication and segregation of the centrosome. [provided by RefSeq, Jul 2008]
UniProt Code:	P41208
NCBI GenInfo Identifier:	729052
NCBI Gene ID:	1069
NCBI Accession:	P41208.1
UniProt Secondary Accession:	P41208,Q53XW1, B2R4T4,
UniProt Related Accession:	P41208
Molecular Weight:	172
NCBI Full Name:	Centrin-2
NCBI Synonym Full Names:	centrin, EF-hand protein, 2
NCBI Official Symbol:	CETN2
NCBI Official Synonym Symbols:	CALT; CEN2
NCBI Protein Information:	centrin-2; caltractin (20kD calcium-binding protein)
UniProt Protein Name:	Centrin-2
UniProt Synonym Protein Names:	Caltractin isoform 1
Protein Family:	Centrin
UniProt Gene Name:	CETN2
UniProt Entry Name:	CETN2_HUMAN

	Western blot analysis of extracts of various cell lines, using CETN2 antibody (CAB5397) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s.
	Western blot analysis of extracts from normal (control) and CETN2 knockout (KO) HeLa cells, using CETN2 antibody (CAB5397) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s.
	Immunofluorescence analysis of A549 cells using CETN2 antibody (CAB5397).
	Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using CETN2 antibody (CAB5397). (Green)GFP-RNF168 fusion protein expression for DNA damage marker.Blue: DAPI for nuclear staining. RNF168 (GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.