Cell Biology Antibodies 5
Anti-CES2 Antibody (CAB1514)
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- SKU:
- CAB1514
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-CES2 Antibody |
| Antibody SKU: | CAB1514 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 310-410 of human CES2 (NP_003860.3). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse |
| Positive Samples: | SW620, Mouse liver |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 310-410 of human CES2 (NP_003860.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | IPGV VDGV FLPR HPQE LLAS ADFQ PVPS IVGV NNNE FGWL IPKV MRIY DTQK EMDR EASQ AALQ KMLT LLML PPTF GDLL REEY IGDN GDPQ TLQA QFQE M |
| Gene ID: | 8824 |
| Uniprot: | O00748 |
| Cellular Location: | Endoplasmic reticulum lumen |
| Calculated MW: | 59kDa/61kDa |
| Observed MW: | 67kDa |
| Synonyms: | CES2, CE-2, CES2A1, PCE-2, iCE |
| Background: | This gene encodes a member of the carboxylesterase large family. The family members are responsible for the hydrolysis or transesterification of various xenobiotics, such as cocaine and heroin, and endogenous substrates with ester, thioester, or amide bonds. They may participate in fatty acyl and cholesterol ester metabolism, and may play a role in the blood-brain barrier system. The protein encoded by this gene is the major intestinal enzyme and functions in intestine drug clearance. Alternatively spliced transcript variants have been found for this gene. |
| UniProt Protein Function: | CES2: Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Shows high catalytic efficiency for hydrolysis of cocaine, 4-methylumbelliferyl acetate, heroin and 6-monoacetylmorphine. Belongs to the type-B carboxylesterase/lipase family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Endoplasmic reticulum; Xenobiotic Metabolism - drug metabolism - other enzymes; EC 3.1.1.84; EC 3.1.1.1; EC 3.1.1.56; Hydrolase Chromosomal Location of Human Ortholog: 16q22.1 Cellular Component: extracellular space; endoplasmic reticulum lumen; endoplasmic reticulum Molecular Function:methylumbelliferyl-acetate deacetylase activity Biological Process: catabolic process |
| NCBI Summary: | This gene encodes a member of the carboxylesterase large family. The family members are responsible for the hydrolysis or transesterification of various xenobiotics, such as cocaine and heroin, and endogenous substrates with ester, thioester, or amide bonds. They may participate in fatty acyl and cholesterol ester metabolism, and may play a role in the blood-brain barrier system. The protein encoded by this gene is the major intestinal enzyme and functions in intestine drug clearance. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Oct 2010] |
| UniProt Code: | O00748 |
| NCBI GenInfo Identifier: | 46576349 |
| NCBI Gene ID: | 8824 |
| NCBI Accession: | O00748.1 |
| UniProt Secondary Accession: | O00748,Q16859, Q5MAB8, Q7Z366, Q8IUP4, Q8TCP8, A8K367 |
| UniProt Related Accession: | O00748 |
| Molecular Weight: | 623 |
| NCBI Full Name: | Cocaine esterase |
| NCBI Synonym Full Names: | carboxylesterase 2 |
| NCBI Official Symbol: | CES2 |
| NCBI Official Synonym Symbols: | iCE; CE-2; PCE-2; CES2A1 |
| NCBI Protein Information: | cocaine esterase; hCE-2; carboxylesterase 2 (intestine, liver); methylumbelliferyl-acetate deacetylase 2; intestinal carboxylesterase; liver carboxylesterase-2 |
| UniProt Protein Name: | Cocaine esterase |
| UniProt Synonym Protein Names: | Carboxylesterase 2 (EC:3.1.1.1); CE-2; hCE-2; Methylumbelliferyl-acetate deacetylase 2 (EC:3.1.1.56) |
| Protein Family: | Liver carboxylesterase |
| UniProt Gene Name: | CES2 |
| UniProt Entry Name: | EST2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CES2 antibody (CAB1514) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
 | Immunohistochemistry of paraffin-embedded human colon using CES2 antibody (CAB1514) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of MCF-7 cells using CES2 antibody (CAB1514). Blue: DAPI for nuclear staining. |
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