Antibody Name:	Anti-CES1 Antibody
Antibody SKU:	CAB1853
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 287-566 of human CES1 (NP_001257.4).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	Mouse liver, A-549, U-937

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 287-566 of human CES1 (NP_001257.4).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	RQKT EEEL LETT LKMK FLSL DLQG DPRE SQPL LGTV IDGM LLLK TPEE LQAE RNFH TVPY MVGI NKQE FGWL IPML MSYP LSEG QLDQ KTAM SLLW KSYP LVCI AKEL IPEA TEKY LGGT DDTV KKKD LFLD LIAD VMFG VPSV IVAR NHRD AGAP TYMY EFQY RPSF SSDM KPKT VIGD HGDE LFSV FGAP FLKE GASE EEIR LSKM VMKF WANF ARNG NPNG EGLP HWPE YNQK EGYL QIGA NTQA AQKL KDKE VAFW TNLF AKKA VEKP PQTE HIEL
Gene ID:	1066
Uniprot:	P23141
Cellular Location:	Endoplasmic reticulum lumen
Calculated MW:	62kDa
Observed MW:	63kDa

Synonyms:

CES1, ACAT, CE-1, CEH, CES2, HMSE, HMSE1, PCE-1, REH, SES1, TGH, hCE-1

Background:

This gene encodes a member of the carboxylesterase large family. The family members are responsible for the hydrolysis or transesterification of various xenobiotics, such as cocaine and heroin, and endogenous substrates with ester, thioester, or amide bonds. They may participate in fatty acyl and cholesterol ester metabolism, and may play a role in the blood-brain barrier system. This enzyme is the major liver enzyme and functions in liver drug clearance. Mutations of this gene cause carboxylesterase 1 deficiency. Three transcript variants encoding three different isoforms have been found for this gene.

UniProt Protein Function:	CES1: Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Hydrolyzes aromatic and aliphatic esters, but has no catalytic activity toward amides or a fatty acyl-CoA ester. Hydrolyzes the methyl ester group of cocaine to form benzoylecgonine. Catalyzes the transesterification of cocaine to form cocaethylene. Displays fatty acid ethyl ester synthase activity, catalyzing the ethyl esterification of oleic acid to ethyloleate. Belongs to the type-B carboxylesterase/lipase family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Hydrolase; Xenobiotic Metabolism - drug metabolism - other enzymes; EC 3.1.1.56; EC 3.1.1.1 Chromosomal Location of Human Ortholog: 16q22.2 Cellular Component: extracellular space; endoplasmic reticulum lumen Molecular Function:methylumbelliferyl-acetate deacetylase activity Biological Process: epithelial cell differentiation; metabolic process; response to toxin
NCBI Summary:	This gene encodes a member of the carboxylesterase large family. The family members are responsible for the hydrolysis or transesterification of various xenobiotics, such as cocaine and heroin, and endogenous substrates with ester, thioester, or amide bonds. They may participate in fatty acyl and cholesterol ester metabolism, and may play a role in the blood-brain barrier system. This enzyme is the major liver enzyme and functions in liver drug clearance. Mutations of this gene cause carboxylesterase 1 deficiency. Three transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]
UniProt Code:	P23141
NCBI GenInfo Identifier:	119576
NCBI Gene ID:	1066
NCBI Accession:	P23141.2
UniProt Related Accession:	P23141
Molecular Weight:	Predicted Molecular Mass: 34.0kDaAccurate Molecular Mass: 35kDa as determined by SDS-PAGE reducing conditions.
NCBI Full Name:	Liver carboxylesterase 1
NCBI Synonym Full Names:	carboxylesterase 1
NCBI Official Symbol:	CES1
NCBI Official Synonym Symbols:	CEH; REH; TGH; ACAT; CE-1; CES2; HMSE; SES1; HMSE1; PCE-1; hCE-1
NCBI Protein Information:	liver carboxylesterase 1
UniProt Protein Name:	Liver carboxylesterase 1
UniProt Synonym Protein Names:	Acyl-coenzyme A:cholesterol acyltransferase; ACAT; Brain carboxylesterase hBr1; Carboxylesterase 1 (EC:3.1.1.1); CE-1; hCE-1; Cocaine carboxylesterase; Egasyn; HMSE; Methylumbelliferyl-acetate deacetylase 1 (EC:3.1.1.56); Monocyte/macrophage serine esterase; Retinyl ester hydrolase; REH; Serine esterase 1; Triacylglycerol hydrolase; TGH
Protein Family:	Liver carboxylesterase
UniProt Gene Name:	CES1
UniProt Entry Name:	EST1_HUMAN

	Western blot analysis of extracts of various cell lines, using CES1 antibody (CAB1853) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunohistochemistry of paraffin-embedded human liver cancer using CES1 antibody (CAB1853) at dilution of 1:100 (40x lens).