Cell Biology Antibodies 6

Anti-CERKL Antibody (CAB15984)

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SKU:
CAB15984
Product Type:
Antibody
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-CERKL Antibody
Antibody SKU:CAB15984
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 359-558 of human CERKL (NP_001025482.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Mouse
Positive Samples:Mouse kidney
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 359-558 of human CERKL (NP_001025482.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:LAEK YRWM SPNQ RRDF AVVK ALAK LKAE DCEI SFLP FNSS DDVQ ERRA QGSP KSDC NDQW QMIQ GQFL NVSI MAIP CLCS VAPR GLAP NTRL NNGS MALI IARN TSRP EFIK HLKR YASV KNQF NFPF VETY TVEE VKVH PRNN TGGY NPEE EEDE TASE NCFP WNVD GDLM EVAS EVHI RLHP RLIS LYGG SMEE MIPK
Gene ID:375298
Uniprot:Q49MI3
Cellular Location:Cytoplasm, Endoplasmic reticulum, Golgi apparatus, Nucleus, nucleolus, nucleolus, trans-Golgi network
Calculated MW:19kDa, 24kDa, 42-62kDa
Observed MW:63kDa
Synonyms:CERKL, RP26
Background:This gene was initially identified as a locus (RP26) associated with an autosomal recessive form of retinitis pigmentosa (arRP) disease. This gene encodes a protein with ceramide kinase-like domains, however, the protein does not phosphorylate ceramide and its target substrate is currently unknown. This protein may be a negative regulator of apoptosis in photoreceptor cells. Mutations in this gene cause a form of retinitis pigmentosa characterized by autosomal recessive cone and rod dystrophy (arCRD). Alternative splicing of this gene results in multiple transcript variants encoding different isoforms and non-coding transcripts.
UniProt Protein Function:CERKL: Has no detectable ceramide-kinase activity. Overexpression of CERKL protects cells from apoptosis in oxidative stress conditions. Defects in CERKL are the cause of retinitis pigmentosa type 26 (RP26). RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP26 inheritance is autosomal recessive. 8 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Nucleolus; Kinase, other

Chromosomal Location of Human Ortholog: 2q31.3

Disease: Retinitis Pigmentosa 26

NCBI Summary:This gene was initially identified as a locus (RP26) associated with an autosomal recessive form of retinitis pigmentosa (arRP) disease. This gene encodes a protein with ceramide kinase-like domains, however, the protein does not phosphorylate ceramide and its target substrate is currently unknown. This protein may be a negative regulator of apoptosis in photoreceptor cells. Mutations in this gene cause a form of retinitis pigmentosa characterized by autosomal recessive cone and rod dystrophy (arCRD). Alternative splicing of this gene results in multiple transcript variants encoding different isoforms and non-coding transcripts.[provided by RefSeq, May 2010]
UniProt Code:Q49MI3
NCBI GenInfo Identifier:84028814
NCBI Gene ID:375298
NCBI Accession:Q49MI3.1
UniProt Secondary Accession:Q49MI3,Q49MH9, Q49MI0, Q49MI1, Q49MI2, Q5DVJ2, Q5DVJ4 Q5DVJ5, Q6UZF6, Q6ZP59, B2RPL2, B4DEY1,
UniProt Related Accession:Q49MI3
Molecular Weight:57,632 Da
NCBI Full Name:Ceramide kinase-like protein
NCBI Synonym Full Names:ceramide kinase like
NCBI Official Symbol:CERKL  
NCBI Official Synonym Symbols:RP26  
NCBI Protein Information:ceramide kinase-like protein
UniProt Protein Name:Ceramide kinase-like protein
Protein Family:Ceramide kinase-like protein
UniProt Gene Name:CERKL  
UniProt Entry Name:CERKL_HUMAN
Anti-CERKL Antibody (CAB15984)Western blot analysis of extracts of mouse kidney, using CERKL antibody (CAB15984) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
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