Cell Biology Antibodies 6

Anti-CERK Antibody (CAB15889)

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SKU:
CAB15889
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-CERK Antibody
Antibody SKU:CAB15889
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 400 to the C-terminus of human CERK (NP_073603.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:LO2, A-549, Mouse testis, Rat thymus
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 400 to the C-terminus of human CERK (NP_073603.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:CRRS PRGL SPAA HLGD GSSD LILI RKCS RFNF LRFL IRHT NQQD QFDF TFVE VYRV KKFQ FTSK HMED EDSD LKEG GKKR FGHI CSSH PSCC CTVS NSSW NCDG EVLH SPAI EVRV HCQL VRLF ARGI EENP KPDS HS
Gene ID:64781
Uniprot:Q8TCT0
Cellular Location:Cytoplasm, Membrane, Peripheral membrane protein
Calculated MW:37kDa/59kDa
Observed MW:60kDa
Synonyms:CERK, LK4, dA59H18.2, dA59H18.3, hCERK
Background:CERK converts ceramide to ceramide 1-phosphate (C1P), a sphingolipid metabolite. Both CERK and C1P have been implicated in various cellular processes, including proliferation, apoptosis, phagocytosis, and inflammation (Kim et al., 2006 [PubMed 16488390]).
UniProt Protein Function:CERK: Catalyzes specifically the phosphorylation of ceramide to form ceramide 1-phosphate. Acts efficiently on natural and analog ceramides (C6, C8, C16 ceramides, and C8-dihydroceramide), to a lesser extent on C2-ceramide and C6-dihydroceramide, but not on other lipids, such as various sphingosines. Binds phosphoinositides.
UniProt Protein Details:

Protein type:EC 2.7.1.138; Kinase, lipid; Lipid Metabolism - sphingolipid

Chromosomal Location of Human Ortholog: 22q13.31

Cellular Component: integral to membrane; plasma membrane

Molecular Function:ceramide kinase activity; magnesium ion binding; protein binding

Biological Process: ceramide metabolic process; glycosphingolipid metabolic process

NCBI Summary:CERK converts ceramide to ceramide 1-phosphate (C1P), a sphingolipid metabolite. Both CERK and C1P have been implicated in various cellular processes, including proliferation, apoptosis, phagocytosis, and inflammation (Kim et al., 2006 [PubMed 16488390]).[supplied by OMIM, Mar 2008]
UniProt Code:Q8TCT0
NCBI GenInfo Identifier:30172885
NCBI Gene ID:64781
NCBI Accession:Q8TCT0.1
UniProt Secondary Accession:Q8TCT0,Q6NX59, Q9BYB3, Q9UGE5, A0JNT4, A8K611,
UniProt Related Accession:Q8TCT0
Molecular Weight:60kDa
NCBI Full Name:Ceramide kinase
NCBI Synonym Full Names:ceramide kinase
NCBI Official Symbol:CERK  
NCBI Official Synonym Symbols:LK4; hCERK; dA59H18.2; dA59H18.3  
NCBI Protein Information:ceramide kinase
UniProt Protein Name:Ceramide kinase
UniProt Synonym Protein Names:Acylsphingosine kinase; Lipid kinase 4; LK4
Protein Family:Ceramide kinase
UniProt Gene Name:CERK  
Anti-CERK Antibody (CAB15889)Western blot analysis of extracts of various cell lines, using CERK antibody (CAB15889) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
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