Cell Biology Antibodies 4

Anti-CERCAM Antibody (CAB14359)

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SKU:
CAB14359
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-CERCAM Antibody
Antibody SKU:CAB14359
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 466-595 of human CERCAM (NP_057258.3).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:U-87MG, 293T
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 466-595 of human CERCAM (NP_057258.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:VVAG YSYW TLAY ALRL AGAR KLLA SQPL RRML PVDE FLPI MFDQ HPNE QYKA HFWP RDLV AFSA QPLL AAPT HYAG DAEW LSDT ETSS PWDD DSGR LISW SGSQ KTLR SPRL DLTG SSGH SLQP QPRD EL
Gene ID:51148
Uniprot:Q5T4B2
Cellular Location:Endoplasmic reticulum lumen
Calculated MW:59kDa/67kDa
Observed MW:75kDa
Synonyms:CERCAM, CEECAM1, GLT25D3
Background:
UniProt Protein Function:CERCAM: Probable cell adhesion protein involved in leukocyte transmigration across the blood-brain barrier. Has apparently no beta-galactosyltransferase activity. Belongs to the glycosyltransferase 25 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:EC 2.-.-.-; Transferase; Cell adhesion; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 9q34.11

Biological Process: cell adhesion; cell motility; leukocyte adhesion

UniProt Code:Q5T4B2
NCBI GenInfo Identifier:74744901
NCBI Gene ID:51148
NCBI Accession:Q5T4B2.1
UniProt Secondary Accession:Q5T4B2,Q0VDF3, Q2VPJ4, Q4KMP2, Q5T4B1, Q8N107, Q96EZ5 Q9P226, Q9UMW5, A7MD00, C4AMA2,
UniProt Related Accession:Q5T4B2
Molecular Weight:59,100 Da
NCBI Full Name:Probable inactive glycosyltransferase 25 family member 3
NCBI Synonym Full Names:cerebral endothelial cell adhesion molecule
NCBI Official Symbol:CERCAM  
NCBI Official Synonym Symbols:CEECAM1; GLT25D3  
NCBI Protein Information:probable inactive glycosyltransferase 25 family member 3
UniProt Protein Name:Probable inactive glycosyltransferase 25 family member 3
UniProt Synonym Protein Names:Cerebral endothelial cell adhesion molecule
Protein Family:Probable inactive glycosyltransferase 25 family
UniProt Gene Name:CERCAM  
UniProt Entry Name:GT253_HUMAN
Anti-CERCAM Antibody (CAB14359)Western blot analysis of extracts of various cell lines, using CERCAM antibody (CAB14359) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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