Cell Cycle Antibodies 2
Anti-CEP55 Antibody (CAB8229)
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- SKU:
- CAB8229
- Product Type:
- Antibody
- Applications:
- WB
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Cycle
Description
| Antibody Name: | CEP55 Rabbit Polyclonal Antibody |
| Antibody SKU: | CAB8229 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human CEP55 (NP_060601.3). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | TE-1 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human CEP55 (NP_060601.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MSSR STKD LIKS KWGS KPSN SKSE TTLE KLKG EIAH LKTS VDEI TSGK GKLT DKER HRLL EKIR VLEA EKEK NAYQ LTEK DKEI QRLR DQLK ARYS TTAL LEQL EETT REGE RREQ VLKA LSEE KDVL KQQL SAAT SRIA ELES KTNT LRLS QTVA PNCF NSSI NNIH EMEI QLKD ALEK NQQW LVYD QQRE VYVK GLLA KIFE LEKK TETA AHSL PQQT KKPE SEGY LQEE KQKC YNDL LASA KKDL EVER QTIT QLSF |
| Gene ID: | 55165 |
| Uniprot: | Q53EZ4 |
| Cellular Location: | Cleavage furrow, Cytoplasm, Midbody, centriole, centrosome, cytoskeleton, microtubule organizing center |
| Calculated MW: | 46kDa/54kDa |
| Observed MW: | 60kDa |
| Synonyms: | CEP55, C10orf3, CT111, URCC6 |
| Background: |
| UniProt Protein Function: | CEP55: Plays a role in mitotic exit and cytokinesis. Not required for microtubule nucleation. Recruits PDCD6IP and TSG101 to midbody during cytokinesis. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Cancer Testis Antigen (CTA); Cell cycle regulation Chromosomal Location of Human Ortholog: 10q23.33 Cellular Component: centrosome; membrane; midbody Molecular Function:protein binding Biological Process: cell separation during cytokinesis; cytokinesis after mitosis; establishment of protein localization; mitotic metaphase plate congression; nuclear organization and biogenesis |
| UniProt Code: | Q53EZ4 |
| NCBI GenInfo Identifier: | 296439403 |
| NCBI Gene ID: | 55165 |
| NCBI Accession: | Q53EZ4.3 |
| UniProt Secondary Accession: | Q53EZ4,Q32WF5, Q3MV20, Q5VY28, Q6N034, Q96H32, Q9NVS7 B2RDG8, |
| UniProt Related Accession: | Q53EZ4 |
| Molecular Weight: | 46,857 Da |
| NCBI Full Name: | Centrosomal protein of 55 kDa |
| NCBI Synonym Full Names: | centrosomal protein 55 |
| NCBI Official Symbol: | CEP55 |
| NCBI Official Synonym Symbols: | CT111; URCC6; C10orf3 |
| NCBI Protein Information: | centrosomal protein of 55 kDa |
| UniProt Protein Name: | Centrosomal protein of 55 kDa |
| UniProt Synonym Protein Names: | Up-regulated in colon cancer 6 |
| Protein Family: | Centrosomal protein |
| UniProt Gene Name: | CEP55 |
| UniProt Entry Name: | CEP55_HUMAN |
 | Western blot analysis of extracts of THP-1 cells, using CEP55 antibody (CAB8229) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s. |
 | Immunofluorescence analysis of L929 cells using CEP55 antibody (CAB8229) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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