Name: Anti-CENPE Antibody (CAB15263)
Brand: Cell Cycle Antibodies 1
SKU: CAB15263
Availability: OutOfStock

Antibody Name:	Anti-CENPE Antibody
Antibody SKU:	CAB15263
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 2542-2701 of human CENPE (NP_001804.2).

Application:	WB
Recommended Dilution:	WB 1:200 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-87MG, HeLa, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 2542-2701 of human CENPE (NP_001804.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	ALIL KSEH IRLE KEIS KLKQ QNEQ LIKQ KNEL LSNN QHLS NEVK TWKE RTLK REAH KQVT CENS PKSP KVTG TASK KKQI TPSQ CKER NLQD PVPK ESPK SCFF DSRS KSLP SPHP VRYF DNSS LGLC PEVQ NAGA ESVD SQPG PWHA SSGK DVPE CKTQ
Gene ID:	1062
Uniprot:	Q02224
Cellular Location:	Chromosome, Cytoplasm, centromere, cytoskeleton, kinetochore, spindle
Calculated MW:	301kDa/312kDa/316kDa
Observed MW:	270kDa

Synonyms:

CENPE, CENP-E, KIF10, MCPH13, PPP1R61

Background:

Centrosome-associated protein E (CENPE) is a kinesin-like motor protein that accumulates in the G2 phase of the cell cycle. Unlike other centrosome-associated proteins, it is not present during interphase and first appears at the centromere region of chromosomes during prometaphase. This protein is required for stable spindle microtubule capture at kinetochores which is a necessary step in chromosome alignment during prometaphase. This protein also couples chromosome position to microtubule depolymerizing activity. Alternative splicing results in multiple transcript variants encoding distinct protein isoforms.

UniProt Protein Function:	CENPE: Essential for the maintenance of chromosomal stability through efficient stabilization of microtubule capture at kinetochores. Plays a key role in the movement of chromosomes toward the metaphase plate during mitosis. Is a slow plus end- directed motor whose activity is essential for metaphase chromosome alignment. Couples chromosome position to microtubule depolymerizing activity. The highly processive microtubule- dependent motor activity of CENPE serves to power chromosome congression and provides a flexible, motile tether linking kinetochores to dynamic spindle microtubules. Necessary for the mitotic checkpoint signal at individual kinetochores to prevent aneuploidy due to single chromosome loss. Required for the efficient recruitment of BUBR1, MAD1 and MAD2 to attached and newly unattached kinetochores. Stimulates mammalian BUBR1 kinase activity. Accumulates just before mitosis at the G2 phase of the cell cycle. Belongs to the kinesin-like protein family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Microtubule-binding; Motor Chromosomal Location of Human Ortholog: 4q24 Cellular Component: chromosome; chromosome, centromeric region; cytosol; kinesin complex; kinetochore; kinetochore microtubule; membrane; microtubule; microtubule cytoskeleton; midbody; nucleus; outer kinetochore of condensed chromosome Molecular Function:ATP binding; ATPase activity; kinetochore binding; microtubule binding; microtubule motor activity; protein binding Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class II; attachment of mitotic spindle microtubules to kinetochore; cell division; chromosome segregation; kinetochore assembly; metaphase plate congression; microtubule-based movement; mitotic cell cycle; mitotic chromosome movement towards spindle pole; mitotic metaphase plate congression; mitotic spindle organization and biogenesis; multicellular organism development; positive regulation of protein kinase activity; regulation of mitotic metaphase/anaphase transition; retrograde vesicle-mediated transport, Golgi to ER; sister chromatid cohesion Disease: Microcephaly 13, Primary, Autosomal Recessive
NCBI Summary:	Centrosome-associated protein E (CENPE) is a kinesin-like motor protein that accumulates in the G2 phase of the cell cycle. Unlike other centrosome-associated proteins, it is not present during interphase and first appears at the centromere region of chromosomes during prometaphase. This protein is required for stable spindle microtubule capture at kinetochores which is a necessary step in chromosome alignment during prometaphase. This protein also couples chromosome position to microtubule depolymerizing activity. Alternative splicing results in multiple transcript variants encoding distinct protein isoforms. [provided by RefSeq, Nov 2014]
UniProt Code:	Q02224
NCBI GenInfo Identifier:	160358869
NCBI Gene ID:	1062
NCBI Accession:	Q02224.2
UniProt Secondary Accession:	Q02224,Q4LE75, A6NKY9, A8K2U7,
UniProt Related Accession:	Q02224
Molecular Weight:	301,789 Da
NCBI Full Name:	Centromere-associated protein E
NCBI Synonym Full Names:	centromere protein E
NCBI Official Symbol:	CENPE
NCBI Official Synonym Symbols:	KIF10; CENP-E; MCPH13; PPP1R61
NCBI Protein Information:	centromere-associated protein E
UniProt Protein Name:	Centromere-associated protein E
UniProt Synonym Protein Names:	Centromere protein E; CENP-E; Kinesin-7
Protein Family:	Centromere-associated protein
UniProt Gene Name:	CENPE

Western blot analysis of extracts of various cell lines, using CENPE Rabbit pAb (CAB15263) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

ELISA Kits

Human CENPE (Centromere Protein E) ELISA Kit (HUES01839)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)