Cell Cycle Antibodies 1

Anti-CENPA Antibody (CAB15995)

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SKU:
CAB15995
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Cycle

Description

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Antibody Name:Anti-CENPA Antibody
Antibody SKU:CAB15995
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human CENPA (NP_001800.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:Jurkat
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human CENPA (NP_001800.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MGPR RRSR KPEA PRRR SPSP TPTP GPSR RGPS LGAS SHQH SRRR QGWL KEIR KLQK STHL LIRK LPFS RLAR EICV KFTR GVDF NWQA QALL ALQE AAEA
Gene ID:1058
Uniprot:P49450
Cellular Location:
Calculated MW:13kDa/15kDa
Observed MW:16kDa
Synonyms:CENPA, CENP-A, CenH3
Background:Centromeres are the differentiated chromosomal domains that specify the mitotic behavior of chromosomes. This gene encodes a centromere protein which contains a histone H3 related histone fold domain that is required for targeting to the centromere. Centromere protein A is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle. The protein is a replication-independent histone that is a member of the histone H3 family. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
UniProt Protein Function:CENPA: a basic nuclear protein of the histone H3 family. May act as a core histone necessary for the assembly of centromeres. Contains a histone H3 related histone fold domain that is required for targeting to the centromere. CENPA is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle.
UniProt Protein Details:

Protein type:DNA-binding

Chromosomal Location of Human Ortholog: 2p23.3

Cellular Component: chromosome, pericentric region; condensed nuclear chromosome, pericentric region; cytosol; nucleoplasm; nucleus

Molecular Function:chromatin binding; protein binding

Biological Process: DNA replication-independent nucleosome assembly at centromere; establishment of mitotic spindle orientation; kinetochore assembly; sister chromatid cohesion

NCBI Summary:Centromeres are the differentiated chromosomal domains that specify the mitotic behavior of chromosomes. This gene encodes a centromere protein which contains a histone H3 related histone fold domain that is required for targeting to the centromere. Centromere protein A is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle. The protein is a replication-independent histone that is a member of the histone H3 family. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Nov 2015]
UniProt Code:P49450
NCBI GenInfo Identifier:1345726
NCBI Gene ID:1058
NCBI Accession:P49450.1
UniProt Secondary Accession:P49450,Q53T74, Q9BVW2, D6W544,
UniProt Related Accession:P49450
Molecular Weight:13,001 Da
NCBI Full Name:Histone H3-like centromeric protein A
NCBI Synonym Full Names:centromere protein A
NCBI Official Symbol:CENPA  
NCBI Official Synonym Symbols:CenH3; CENP-A  
NCBI Protein Information:histone H3-like centromeric protein A
UniProt Protein Name:Histone H3-like centromeric protein A
UniProt Synonym Protein Names:Centromere autoantigen A; Centromere protein A; CENP-A
Protein Family:Histone H3-like centromeric protein
UniProt Gene Name:CENPA  
UniProt Entry Name:CENPA_HUMAN
Anti-CENPA Antibody (CAB15995)Western blot analysis of extracts of Jurkat cells, using CENPA antibody (CAB15995) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
Anti-CENPA Antibody (CAB15995)Immunofluorescence analysis of U-2 OS cells using CENPA Polyclonal Antibody (CAB15995) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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