Cell Cycle Antibodies 1
Anti-CENPA Antibody (CAB15995)
(No reviews yet)
Write a Review
- SKU:
- CAB15995
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Cycle
Description
| Antibody Name: | Anti-CENPA Antibody |
| Antibody SKU: | CAB15995 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human CENPA (NP_001800.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Jurkat |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human CENPA (NP_001800.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MGPR RRSR KPEA PRRR SPSP TPTP GPSR RGPS LGAS SHQH SRRR QGWL KEIR KLQK STHL LIRK LPFS RLAR EICV KFTR GVDF NWQA QALL ALQE AAEA |
| Gene ID: | 1058 |
| Uniprot: | P49450 |
| Cellular Location: | |
| Calculated MW: | 13kDa/15kDa |
| Observed MW: | 16kDa |
| Synonyms: | CENPA, CENP-A, CenH3 |
| Background: | Centromeres are the differentiated chromosomal domains that specify the mitotic behavior of chromosomes. This gene encodes a centromere protein which contains a histone H3 related histone fold domain that is required for targeting to the centromere. Centromere protein A is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle. The protein is a replication-independent histone that is a member of the histone H3 family. Alternative splicing results in multiple transcript variants encoding distinct isoforms. |
| UniProt Protein Function: | CENPA: a basic nuclear protein of the histone H3 family. May act as a core histone necessary for the assembly of centromeres. Contains a histone H3 related histone fold domain that is required for targeting to the centromere. CENPA is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle. |
| UniProt Protein Details: | Protein type:DNA-binding Chromosomal Location of Human Ortholog: 2p23.3 Cellular Component: chromosome, pericentric region; condensed nuclear chromosome, pericentric region; cytosol; nucleoplasm; nucleus Molecular Function:chromatin binding; protein binding Biological Process: DNA replication-independent nucleosome assembly at centromere; establishment of mitotic spindle orientation; kinetochore assembly; sister chromatid cohesion |
| NCBI Summary: | Centromeres are the differentiated chromosomal domains that specify the mitotic behavior of chromosomes. This gene encodes a centromere protein which contains a histone H3 related histone fold domain that is required for targeting to the centromere. Centromere protein A is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle. The protein is a replication-independent histone that is a member of the histone H3 family. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Nov 2015] |
| UniProt Code: | P49450 |
| NCBI GenInfo Identifier: | 1345726 |
| NCBI Gene ID: | 1058 |
| NCBI Accession: | P49450.1 |
| UniProt Secondary Accession: | P49450,Q53T74, Q9BVW2, D6W544, |
| UniProt Related Accession: | P49450 |
| Molecular Weight: | 13,001 Da |
| NCBI Full Name: | Histone H3-like centromeric protein A |
| NCBI Synonym Full Names: | centromere protein A |
| NCBI Official Symbol: | CENPA |
| NCBI Official Synonym Symbols: | CenH3; CENP-A |
| NCBI Protein Information: | histone H3-like centromeric protein A |
| UniProt Protein Name: | Histone H3-like centromeric protein A |
| UniProt Synonym Protein Names: | Centromere autoantigen A; Centromere protein A; CENP-A |
| Protein Family: | Histone H3-like centromeric protein |
| UniProt Gene Name: | CENPA |
| UniProt Entry Name: | CENPA_HUMAN |
 | Western blot analysis of extracts of Jurkat cells, using CENPA antibody (CAB15995) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
 | Immunofluorescence analysis of U-2 OS cells using CENPA Polyclonal Antibody (CAB15995) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
View AllClose
Related Products
Anti-Phospho-CENPA-S7 Antibody (CABP1183)
OverviewProduct Name:Phospho-CENPA-S7 Rabbit pAbProduct Code:CABP1183Size:20uL, 50uL, 100uLSynonyms:CENP-A, CenH3Appli
Anti-RANBP3 Antibody (CAB0092)
OverviewProduct Name:RANBP3 Rabbit mAbProduct Code:CAB0092Size:20uL, 50uL, 100uLSynonyms:RANBP3, ran-binding protein 3
Anti-QK1 Antibody (CAB0193)
OverviewProduct Name:QK1 Rabbit mAbProduct Code:CAB0193Size:20uL, 50uL, 100uLSynonyms:QKI, Hqk, QK, QK1, QK3, hqkI, protein quaking
Anti-DEK Antibody (CAB0315)
OverviewProduct Name:DEK Rabbit mAbProduct Code:CAB0315Size:20uL, 50uL, 100uLSynonyms:D6S231EApplications:WB,