Epigenetics & Nuclear Signaling Antibodies 2

Anti-CELF1 Antibody (CAB5958)

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SKU:
CAB5958
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-CELF1 Antibody
Antibody SKU:CAB5958
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 108-483 of human CELF1 (NP_941989.1).
Application:WB IHC
Recommended Dilution:WB 1:200 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:U-87MG, SH-SY5Y, HepG2, NIH/3T3, HeLa, Mouse liver, Mouse testis
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 108-483 of human CELF1 (NP_941989.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:RKLF IGMI SKKC TEND IRVM FSSF GQIE ECRI LRGP DGLS RGCA FVTF TTRA MAQT AIKA MHQA QTME GCSS PMVV KFAD TQKD KEQK RMAQ QLQQ QMQQ ISAA SVWG NLAG LNTL GPQY LALL QQTA SSGN LNTL SSLH PMGG LNAM QLQN LAAL AAAA SAAQ NTPS GTNA LTTS SSPL SVLT SSAG SSPS SSSS NSVN PIAS LGAL QTLA GATA GLNV GSLA GMAA LNGG LGSS GLSN GTGS TMEA LTQA YSGI QQYA AAAL PTLY NQNL LTQQ SIGA AGSQ KEGP EGAN LFIY HLPQ EFGD QDLL QMFM PFGN VVSA KVFI DKQT NLSK CFGF VSYD NPVS AQAA IQSM NGFQ IGMK RLKV QLKR SKND SKPY
Gene ID:10658
Uniprot:Q92879
Cellular Location:Cytoplasm, Nucleus
Calculated MW:50kDa/51kDa/52kDa/55kDa
Observed MW:52kDa
Synonyms:CELF1, BRUNOL2, CUG-BP, CUGBP, CUGBP1, EDEN-BP, NAB50, NAPOR, hNab50
Background:Members of the CELF/BRUNOL protein family contain two N-terminal RNA recognition motif (RRM) domains, one C-terminal RRM domain, and a divergent segment of 160-230 aa between the second and third RRM domains. Members of this protein family regulate pre-mRNA alternative splicing and may also be involved in mRNA editing, and translation. This gene may play a role in myotonic dystrophy type 1 (DM1) via interactions with the dystrophia myotonica-protein kinase (DMPK) gene. Alternative splicing results in multiple transcript variants encoding different isoforms.
UniProt Protein Function:CELF1: RNA-binding protein implicated in the regulation of several post-transcriptional events. Involved in pre-mRNA alternative splicing, mRNA translation and stability. Mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing. Specifically activates exon 5 inclusion of cardiac isoforms of TNNT2 during heart remodeling at the juvenile to adult transition. Acts as both an activator and repressor of a pair of coregulated exons: promotes inclusion of the smooth muscle (SM) exon but exclusion of the non-muscle (NM) exon in actinin pre- mRNAs. Activates SM exon 5 inclusion by antagonizing the repressive effect of PTB. Promotes exclusion of exon 11 of the INSR pre-mRNA. Inhibits, together with HNRNPH1, insulin receptor (IR) pre-mRNA exon 11 inclusion in myoblast. Increases translation and controls the choice of translation initiation codon of CEBPB mRNA. Increases mRNA translation of CEBPB in aging liver. Increases translation of CDKN1A mRNA by antagonizing the repressive effect of CALR3. Mediates rapid cytoplasmic mRNA deadenylation. Recruits the deadenylase PARN to the poly(A) tail of EDEN-containing mRNAs to promote their deadenylation. Required for completion of spermatogenesis. Binds to (CUG)n triplet repeats in the 3'-UTR of transcripts such as DMPK and to Bruno response elements (BREs). Binds to muscle-specific splicing enhancer (MSE) intronic sites flanking the alternative exon 5 of TNNT2 pre-mRNA. Binds to AU-rich sequences (AREs or EDEN-like) localized in the 3'-UTR of JUN and FOS mRNAs. Binds to the IR RNA. Binds to the 5'-region of CDKN1A and CEBPB mRNAs. Binds with the 5'-region of CEBPB mRNA in aging liver. Belongs to the CELF/BRUNOL family. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:RNA-binding; RNA processing; Cell development/differentiation

Chromosomal Location of Human Ortholog: 11p11

Cellular Component: nucleoplasm; membrane; cytoplasm; ribonucleoprotein complex; nucleus

Molecular Function:mRNA binding; protein binding; RNA binding; translation initiation factor binding; BRE binding; translation repressor activity, nucleic acid binding; nucleotide binding

Biological Process: mRNA splice site selection; embryonic development; regulation of RNA splicing; negative regulation of translation; positive regulation of multicellular organism growth; mRNA processing; spermatid development; germ cell development; RNA interference

NCBI Summary:Members of the CELF/BRUNOL protein family contain two N-terminal RNA recognition motif (RRM) domains, one C-terminal RRM domain, and a divergent segment of 160-230 aa between the second and third RRM domains. Members of this protein family regulate pre-mRNA alternative splicing and may also be involved in mRNA editing, and translation. This gene may play a role in myotonic dystrophy type 1 (DM1) via interactions with the dystrophia myotonica-protein kinase (DMPK) gene. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
UniProt Code:Q92879
NCBI GenInfo Identifier:17374605
NCBI Gene ID:10658
NCBI Accession:Q92879.2
UniProt Secondary Accession:Q92879,Q4LE52, Q9NP83, Q9NR06, B4E2U5, D3DQS0, F8W940
UniProt Related Accession:Q92879
Molecular Weight:51,992 Da
NCBI Full Name:CUGBP Elav-like family member 1
NCBI Synonym Full Names:CUGBP, Elav-like family member 1
NCBI Official Symbol:CELF1  
NCBI Official Synonym Symbols:CUGBP; NAB50; NAPOR; CUG-BP; CUGBP1; hNab50; BRUNOL2; EDEN-BP  
NCBI Protein Information:CUGBP Elav-like family member 1; CELF-1; CUG-BP1; bruno-like 2; EDEN-BP homolog; bruno-like protein 2; CUG RNA-binding protein; deadenylation factor CUG-BP; RNA-binding protein BRUNOL-2; CUG-BP- and ETR-3-like factor 1; CUG triplet repeat RNA-binding protein 1; CUG triplet repeat, RNA binding protein 1; CUG triplet repeat, RNA-binding protein 1; embryo deadenylation element binding protein; 50 kDa nuclear polyadenylated RNA-binding protein; nuclear polyadenylated RNA-binding protein, 50-kD; embryo deadenylation element-binding protein homolog
UniProt Protein Name:CUGBP Elav-like family member 1
UniProt Synonym Protein Names:50 kDa nuclear polyadenylated RNA-binding protein; Bruno-like protein 2; CUG triplet repeat RNA-binding protein 1; CUG-BP1; CUG-BP- and ETR-3-like factor 1; Deadenylation factor CUG-BP; Embryo deadenylation element-binding protein homolog; EDEN-BP homolog; RNA-binding protein BRUNOL-2
Protein Family:CUGBP Elav-like family
UniProt Gene Name:CELF1  
UniProt Entry Name:CELF1_HUMAN
Anti-CELF1 Antibody (CAB5958)Western blot analysis of extracts of various cell lines, using CELF1 antibody (CAB5958) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
Anti-CELF1 Antibody (CAB5958)Immunohistochemistry of paraffin-embedded Mouse brain using CELF1 Rabbit pAb (CAB5958) at dilution of 1:100 (40x lens).
Anti-CELF1 Antibody (CAB5958)Immunohistochemistry of paraffin-embedded Rat brain using CELF1 Rabbit pAb (CAB5958) at dilution of 1:100 (40x lens).
Anti-CELF1 Antibody (CAB5958)Immunohistochemistry of paraffin-embedded Human colon using CELF1 Rabbit pAb (CAB5958) at dilution of 1:100 (40x lens).
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OverviewProduct Name:CELF1 Rabbit mAbProduct Code:CAB1150Size:20uL, 50uL, 100uLSynonyms:BRUNOL2, CUG-BP, CUGBP, CUGBP1, EDEN-BP, NAB50,