Cell Cycle Antibodies 1

Anti-CDKN3 Antibody (CAB2061)

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SKU:
CAB2061
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Cycle

Description

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Antibody Name:Anti-CDKN3 Antibody
Antibody SKU:CAB2061
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-172 of human CDKN3 (NP_001124323.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse
Positive Samples:Mouse liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-172 of human CDKN3 (NP_001124323.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MKPP SSIQ TSCK FKDV RRNV QKDT EELK SCGI QDIF VFCT RGEL SKYR VPNL LDLY QQCG IITH HHPI ADGG TPDI ASCC EIME ELTT CLKN YRKT LIHC YGGL GRSC LVAA CLLL YLSD TISP EQAI DSLR DLRG SGAI QTIK QYNY LHEF RDKL AAHL SSRD SQSR SVSR
Gene ID:1033
Uniprot:Q16667
Cellular Location:Cytoplasm, perinuclear region
Calculated MW:19kDa/23kDa
Observed MW:23kDa
Synonyms:CDKN3, CDI1, CIP2, KAP, KAP1
Background:The protein encoded by this gene belongs to the dual specificity protein phosphatase family. It was identified as a cyclin-dependent kinase inhibitor, and has been shown to interact with, and dephosphorylate CDK2 kinase, thus prevent the activation of CDK2 kinase. This gene was reported to be deleted, mutated, or overexpressed in several kinds of cancers. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:CDKN3: May play a role in cell cycle regulation. Dual specificity phosphatase active toward substrates containing either phosphotyrosine or phosphoserine residues. Dephosphorylates CDK2 at 'Thr-160' in a cyclin-dependent manner. Defects in CDKN3 are found in patients with hepatocellular carcinoma (HCC). Belongs to the protein-tyrosine phosphatase family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Motility/polarity/chemotaxis; Protein phosphatase, dual-specificity; EC 3.1.3.16; Cell cycle regulation; EC 3.1.3.48

Chromosomal Location of Human Ortholog: 14q22

Cellular Component: perinuclear region of cytoplasm; cytoplasm

Molecular Function:protein binding; protein tyrosine/serine/threonine phosphatase activity; protein tyrosine phosphatase activity; protein serine/threonine phosphatase activity

Biological Process: negative regulation of cell proliferation; regulation of cyclin-dependent protein kinase activity; cell cycle arrest; G1/S transition of mitotic cell cycle

Disease: Hepatocellular Carcinoma

NCBI Summary:The protein encoded by this gene belongs to the dual specificity protein phosphatase family. It was identified as a cyclin-dependent kinase inhibitor, and has been shown to interact with, and dephosphorylate CDK2 kinase, thus prevent the activation of CDK2 kinase. This gene was reported to be deleted, mutated, or overexpressed in several kinds of cancers. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]
UniProt Code:Q16667
NCBI GenInfo Identifier:2499769
NCBI Gene ID:1033
NCBI Accession:Q16667.1
UniProt Related Accession:Q16667
Molecular Weight:23805 Da
NCBI Full Name:Cyclin-dependent kinase inhibitor 3
NCBI Synonym Full Names:cyclin dependent kinase inhibitor 3
NCBI Official Symbol:CDKN3  
NCBI Official Synonym Symbols:KAP; CDI1; CIP2; KAP1  
NCBI Protein Information:cyclin-dependent kinase inhibitor 3
UniProt Protein Name:Cyclin-dependent kinase inhibitor 3
UniProt Synonym Protein Names:CDK2-associated dual-specificity phosphatase; Cyclin-dependent kinase interactor 1; Cyclin-dependent kinase-interacting protein 2; Kinase-associated phosphatase
Protein Family:Cyclin-dependent kinase inhibitor
UniProt Gene Name:CDKN3  
UniProt Entry Name:CDKN3_HUMAN
Anti-CDKN3 Antibody (CAB2061)Western blot analysis of extracts of mouse liver, using CDKN3 antibody (CAB2061) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Anti-CDKN3 Antibody (CAB2061)Immunofluorescence analysis of MCF-7 cells using CDKN3 antibody (CAB2061). Blue: DAPI for nuclear staining.
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