Description
| Antibody Name: | Anti-CDKN2C/p18-INK4C Antibody |
| Antibody SKU: | CAB2042 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-168 of human CDKN2C/p18-INK4C (NP_523240.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | 293T, HeLa, MCF7, A-549, Mouse testis, Rat skeletal muscle |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-168 of human CDKN2C/p18-INK4C (NP_523240.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAEP WGNE LASA AARG DLEQ LTSL LQNN VNVN AQNG FGRT ALQV MKLG NPEI ARRL LLRG ANPD LKDR TGFA VIHD AARA GFLD TLQT LLEF QADV NIED NEGN LPLH LAAK EGHL RVVE FLVK HTAS NVGH RNHK GDTA CDLA RLYG RNEV VSLM QANG AGGA TNLQ |
| Gene ID: | 1031 |
| Uniprot: | P42773 |
| Cellular Location: | |
| Calculated MW: | 18kDa |
| Observed MW: | 18kDa |
| Synonyms: | CDKN2C, INK4C, p18, p18-INK4C |
| Background: | The protein encoded by this gene is a member of the INK4 family of cyclin-dependent kinase inhibitors. This protein has been shown to interact with CDK4 or CDK6, and prevent the activation of the CDK kinases, thus function as a cell growth regulator that controls cell cycle G1 progression. Ectopic expression of this gene was shown to suppress the growth of human cells in a manner that appears to correlate with the presence of a wild-type RB1 function. Studies in the knockout mice suggested the roles of this gene in regulating spermatogenesis, as well as in suppressing tumorigenesis. Two alternatively spliced transcript variants of this gene, which encode an identical protein, have been reported. |
| UniProt Protein Function: | Interacts strongly with CDK6, weakly with CDK4. Inhibits cell growth and proliferation with a correlated dependence on endogenous retinoblastoma protein RB. |
| NCBI Summary: | The protein encoded by this gene is a member of the INK4 family of cyclin-dependent kinase inhibitors. This protein has been shown to interact with CDK4 or CDK6, and prevent the activation of the CDK kinases, thus function as a cell growth regulator that controls cell cycle G1 progression. Ectopic expression of this gene was shown to suppress the growth of human cells in a manner that appears to correlate with the presence of a wild-type RB1 function. Studies in the knockout mice suggested the roles of this gene in regulating spermatogenesis, as well as in suppressing tumorigenesis. Two alternatively spliced transcript variants of this gene, which encode an identical protein, have been reported. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P42773 |
| NCBI GenInfo Identifier: | 1168870 |
| NCBI Gene ID: | 1031 |
| NCBI Accession: | P42773.1 |
| UniProt Secondary Accession: | P42773,Q8TB83, |
| UniProt Related Accession: | P42773 |
| Molecular Weight: | 18kDa |
| NCBI Full Name: | Cyclin-dependent kinase 4 inhibitor C |
| NCBI Synonym Full Names: | cyclin dependent kinase inhibitor 2C |
| NCBI Official Symbol: | CDKN2C |
| NCBI Official Synonym Symbols: | p18; INK4C; p18-INK4C |
| NCBI Protein Information: | cyclin-dependent kinase 4 inhibitor C |
| UniProt Protein Name: | Cyclin-dependent kinase 4 inhibitor C |
| UniProt Synonym Protein Names: | Cyclin-dependent kinase 6 inhibitor; p18-INK4c; p18-INK6 |
| Protein Family: | Cyclin-dependent kinase 4 inhibitor |
| UniProt Gene Name: | CDKN2C |
 | Western blot analysis of extracts of various cell lines, using CDKN2C/p18-INK4C antibody (CAB2042) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
 | Immunohistochemistry of paraffin-embedded rat lung using CDKN2C/p18-INK4C antibody (CAB2042) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human stomach using CDKN2C/p18-INK4C antibody (CAB2042) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse lung using CDKN2C/p18-INK4C antibody (CAB2042) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of HeLa cells using CDKN2C/p18-INK4C antibody (CAB2042). Blue: DAPI for nuclear staining. |
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