Cell Cycle Antibodies 1
Anti-CDKN1C/p57 Kip2 Antibody (CAB2060)
- SKU:
- CAB2060
- Product Type:
- Antibody
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Cycle
Description
| Antibody Name: | Anti-CDKN1C/p57 Kip2 Antibody |
| Antibody SKU: | CAB2060 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human CDKN1C/p57 Kip2 (NP_000067.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Mouse, Rat |
| Positive Samples: | Mouse kidney, Rat kidney |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human CDKN1C/p57 Kip2 (NP_000067.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MSDA SLRS TSTM ERLV ARGT FPVL VRTS ACRS LFGP VDHE ELSR ELQA RLAE LNAE DQNR WDYD FQQD MPLR GPGR LQWT EVDS DSVP AFYR ETVQ VGRC |
| Gene ID: | 1028 |
| Uniprot: | P49918 |
| Cellular Location: | Nucleus |
| Calculated MW: | 31kDa/32kDa |
| Observed MW: | 55kDa |
| Synonyms: | CDKN1C, BWCR, BWS, KIP2, WBS, p57, p57Kip2 |
| Background: | This gene is imprinted, with preferential expression of the maternal allele. The encoded protein is a tight-binding, strong inhibitor of several G1 cyclin/Cdk complexes and a negative regulator of cell proliferation. Mutations in this gene are implicated in sporadic cancers and Beckwith-Wiedemann syndorome, suggesting that this gene is a tumor suppressor candidate. Three transcript variants encoding two different isoforms have been found for this gene. |
| UniProt Protein Function: | p57Kip2: Potent tight-binding inhibitor of several G1 cyclin/CDK complexes (cyclin E-CDK2, cyclin D2-CDK4, and cyclin A-CDK2) and, to lesser extent, of the mitotic cyclin B-CDC2. Negative regulator of cell proliferation. May play a role in maintenance of the non- proliferative state throughout life. Expressed in the heart, brain, lung, skeletal muscle, kidney, pancreas and testis. High levels are seen in the placenta while low levels are seen in the liver. Belongs to the CDI family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Inhibitor; Tumor suppressor; Nuclear receptor co-regulator; Cell cycle regulation Chromosomal Location of Human Ortholog: 11p15.5 Cellular Component: cytoplasm; nucleus Molecular Function:cyclin-dependent protein kinase inhibitor activity; protein binding Biological Process: negative regulation of kinase activity; camera-type eye development; adrenal gland development; multicellular organism growth; positive regulation of transcription, DNA-dependent; regulation of mitotic cell cycle; positive regulation of transforming growth factor beta receptor signaling pathway; negative regulation of transcription from RNA polymerase II promoter; uterus development; neuron maturation; negative regulation of phosphorylation; myeloid cell differentiation; kidney development; cell cycle arrest; negative regulation of transcription, DNA-dependent; skeletal development; aging; negative regulation of epithelial cell proliferation Disease: Intrauterine Growth Retardation, Metaphyseal Dysplasia, Adrenal Hypoplasia Congenita, And Genital Anomalies; Beckwith-wiedemann Syndrome |
| NCBI Summary: | This gene is imprinted, with preferential expression of the maternal allele. The encoded protein is a tight-binding, strong inhibitor of several G1 cyclin/Cdk complexes and a negative regulator of cell proliferation. Mutations in this gene are implicated in sporadic cancers and Beckwith-Wiedemann syndorome, suggesting that this gene is a tumor suppressor candidate. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Oct 2010] |
| UniProt Code: | P49918 |
| NCBI GenInfo Identifier: | 1705731 |
| NCBI Gene ID: | 1028 |
| NCBI Accession: | P49918.1 |
| UniProt Related Accession: | P49918 |
| Molecular Weight: | 316 |
| NCBI Full Name: | Cyclin-dependent kinase inhibitor 1C |
| NCBI Synonym Full Names: | cyclin-dependent kinase inhibitor 1C (p57, Kip2) |
| NCBI Official Symbol: | CDKN1C |
| NCBI Official Synonym Symbols: | BWS; WBS; p57; BWCR; KIP2; p57Kip2 |
| NCBI Protein Information: | cyclin-dependent kinase inhibitor 1C; cyclin-dependent kinase inhibitor p57 |
| UniProt Protein Name: | Cyclin-dependent kinase inhibitor 1C |
| UniProt Synonym Protein Names: | Cyclin-dependent kinase inhibitor p57; p57Kip2 |
| Protein Family: | Cyclin-dependent kinase inhibitor |
| UniProt Gene Name: | CDKN1C |
| UniProt Entry Name: | CDN1C_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CDKN1C/p57 Kip2 antibody (CAB2060) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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