Antibody Name:	Anti-CDKN1B/p27KIP1 Antibody [KO Validated]
Antibody SKU:	CAB0290
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-198 of human CDKN1B/p27KIP1 (NP_004055.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:20 - 1:50
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, MCF7, HepG2

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-198 of human CDKN1B/p27KIP1 (NP_004055.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSNV RVSN GSPS LERM DARQ AEHP KPSA CRNL FGPV DHEE LTRD LEKH CRDM EEAS QRKW NFDF QNHK PLEG KYEW QEVE KGSL PEFY YRPP RPPK GACK VPAQ ESQD VSGS RPAA PLIG APAN SEDT HLVD PKTD PSDS QTGL AEQC AGIR KRPA TDDS STQN KRAN RTEE NVSD GSPN AGSV EQTP KKPG LRRR QT
Gene ID:	1027
Uniprot:	P46527
Cellular Location:	Cytoplasm, Endosome, Nucleus
Calculated MW:	22kDa
Observed MW:	27kDa

Synonyms:

CDKN1B, CDKN4, KIP1, MEN1B, MEN4, P27KIP1, p27 KIP 1

Background:

This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Mutations in this gene are associated with multiple endocrine neoplasia type IV (MEN4).

UniProt Protein Function:	p27Kip1: a cell-cycle regulatory protein that Interacts with cyclin-CDK2 and -CDK4, inhibiting cell cycle progression at G1. May mediate TGF beta-induced g1 arrest. Its degradation is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state.
UniProt Protein Details:	Protein type:Protein kinase, regulatory subunit; Cell cycle regulation; Oncoprotein; Inhibitor Chromosomal Location of Human Ortholog: 12p13.1-p12 Cellular Component: nucleoplasm; cytoplasm; nucleus; cytosol; endosome Molecular Function:cyclin-dependent protein kinase inhibitor activity; protein binding; chaperone binding; protein complex binding; caspase activator activity; Hsp70 protein binding; protein phosphatase binding; transforming growth factor beta receptor, cytoplasmic mediator activity Biological Process: negative regulation of kinase activity; response to peptide hormone stimulus; nerve growth factor receptor signaling pathway; positive regulation of microtubule polymerization; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; response to estradiol stimulus; negative regulation of cell proliferation; sensory perception of sound; positive regulation of cell proliferation; response to glucose stimulus; negative regulation of mitotic cell cycle; cell cycle arrest; inner ear development; potassium ion transport; epidermal growth factor receptor signaling pathway; caspase activation; response to drug; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; negative regulation of cyclin-dependent protein kinase activity; negative regulation of cell motility; response to amino acid stimulus; response to cadmium ion; response to hypoxia; positive regulation of protein catabolic process; autophagic cell death; innate immune response; negative regulation of phosphorylation; negative regulation of cell growth; regulation of cyclin-dependent protein kinase activity; mitotic cell cycle; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis; G1/S transition of mitotic cell cycle Disease: Multiple Endocrine Neoplasia, Type Iv
NCBI Summary:	This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Mutations in this gene are associated with multiple endocrine neoplasia type IV (MEN4). [provided by RefSeq, Apr 2014]
UniProt Code:	P46527
NCBI GenInfo Identifier:	1168871
NCBI Gene ID:	1027
NCBI Accession:	P46527.1
UniProt Secondary Accession:	P46527,Q16307, Q5U0H2, Q9BUS6,
UniProt Related Accession:	P46527
Molecular Weight:	Calculated: 22kDaObserved: 26kDa
NCBI Full Name:	Cyclin-dependent kinase inhibitor 1B
NCBI Synonym Full Names:	cyclin-dependent kinase inhibitor 1B (p27, Kip1)
NCBI Official Symbol:	CDKN1B
NCBI Official Synonym Symbols:	KIP1; MEN4; CDKN4; MEN1B; P27KIP1
NCBI Protein Information:	cyclin-dependent kinase inhibitor 1B
UniProt Protein Name:	Cyclin-dependent kinase inhibitor 1B
UniProt Synonym Protein Names:	Cyclin-dependent kinase inhibitor p27; p27Kip1
Protein Family:	Cyclin-dependent kinase inhibitor
UniProt Gene Name:	CDKN1B
UniProt Entry Name:	CDN1B_HUMAN

	Western blot analysis of extracts from normal (control) and CDKN1B/p27KIP1 knockout (KO) HeLa cells, using CDKN1B/p27KIP1 antibody (CAB0290) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3min.
	Western blot analysis of extracts of various cell lines, using CDKN1B/p27KIP1 Rabbit pAb (CAB0290) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3min.
	Immunohistochemistry of paraffin-embedded rat brain using CDKN1B/p27KIP1 Antibody (CAB0290) at dilution of 1:200 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse kidney using CDKN1B/p27KIP1 Antibody (CAB0290) at dilution of 1:200 (40x lens).
	Immunohistochemistry of paraffin-embedded rat brain using CDKN1B/p27KIP1 Antibody (CAB0290) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
Anti-CDKN1B/p27KIP1 Antibody (CAB13613)	Human P27 (P27 Protein) ELISA Kit (HUES02035)
Anti-CDKN1B/p27KIP1 Antibody (CAB15632)	p27 Kip1 (Phospho-Ser10) Colorimetric Cell-Based ELISA Kit
Anti-CDKN1B/p27KIP1 Antibody (CAB16633)[KO Validated]	p27 Kip1 Colorimetric Cell-Based ELISA Kit
Anti-CDKN1B/p27KIP1 Antibody (CAB16722)	p27 Kip1 (Phospho-Thr198) Colorimetric Cell-Based ELISA Kit
Anti-Phospho-CDKN1B-T187 Antibody (CABP0328)	p27 Kip1 (Phospho-Thr187) Colorimetric Cell-Based ELISA Kit