Name: Anti-CDK5RAP3 Antibody (CAB13128)
Brand: Immunology Antibodies 1
SKU: CAB13128
Availability: OutOfStock

Antibody Name:	Anti-CDK5RAP3 Antibody
Antibody SKU:	CAB13128
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human CDK5RAP3 (NP_788276.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:	Human
Positive Samples:	U-87MG, 293T, LO2

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human CDK5RAP3 (NP_788276.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MEDH QHVP IDIQ TSKL LDWL VDRR HCSL KWQS LVLT IREK INAA IQDM PESE EIAQ LLSG SYIH YFHC LRIL DLLK GTEA STKN IFGR YSSQ RMKD WQEI IALY EKDN TYLV ELSS LLVR NVNY EIPS LKKQ IAKC QQLQ QEYS RKEE ECQA GAAE MREQ FYHS CKQY GITG ENVR GELL ALVK DLPS QLAE IGAA AQQS LGEA IDVY QASV GFVC ESPT EQVL PMLR FVQK RGNS TVYE WRTG TEPS VVER PHLE ELPE QVAE DAID WGDF GVEA VSEG TDSG ISAE AAGI DWGI FPES
Gene ID:	80279
Uniprot:	Q96JB5
Cellular Location:	Cytoplasm, Nucleus, centrosome, cytoskeleton, microtubule organizing center
Calculated MW:	31kDa/46kDa/56kDa/59kDa
Observed MW:	65kDa

Synonyms:	CDK5RAP3, C53, HSF-27, IC53, LZAP, MST016, OK/SW-cl.114, PP1553
Background:	This gene encodes a protein that has been reported to function in signaling pathways governing transcriptional regulation and cell cycle progression. It may play a role in tumorigenesis and metastasis. A pseudogene of this gene is located on the long arm of chromosome 20. Alternative splicing results in multiple transcript variants that encode different isoforms.

UniProt Protein Function:	CDK5RAP3: Potential regulator of CDK5 activity. May be involved in cell proliferation. Regulates CDK5 activity via its interaction with CDK5R1. Belongs to the CDK5RAP3 family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Cell cycle regulation Chromosomal Location of Human Ortholog: 17q21.32 Cellular Component: protein complex; membrane; cytoplasm; nucleolus; endomembrane system; nucleus Molecular Function:cyclin binding; protein binding; protein kinase binding Biological Process: cell proliferation; regulation of neuron differentiation; positive regulation of protein ubiquitination; inhibition of NF-kappaB transcription factor; regulation of mitotic cell cycle; positive regulation of transcription from RNA polymerase II promoter; brain development; regulation of cyclin-dependent protein kinase activity
NCBI Summary:	This gene encodes a protein that has been reported to function in signaling pathways governing transcriptional regulation and cell cycle progression. It may play a role in tumorigenesis and metastasis. A pseudogene of this gene is located on the long arm of chromosome 20. Alternative splicing results in multiple transcript variants that encode different isoforms. [provided by RefSeq, May 2013]
UniProt Code:	Q96JB5
NCBI GenInfo Identifier:	32129444
NCBI Gene ID:	80279
NCBI Accession:	Q96JB5.2
UniProt Secondary Accession:	Q96JB5,Q53FA2, Q9H3F8, Q9H8G0, Q9HBR9, B7Z6N4, D3DTU1 D3DTU2, F5H3I5,
UniProt Related Accession:	Q96JB5
Molecular Weight:	506
NCBI Full Name:	CDK5 regulatory subunit-associated protein 3
NCBI Synonym Full Names:	CDK5 regulatory subunit associated protein 3
NCBI Official Symbol:	CDK5RAP3
NCBI Official Synonym Symbols:	C53; IC53; LZAP; HSF-27; MST016; PP1553; OK/SW-cl.114
NCBI Protein Information:	CDK5 regulatory subunit-associated protein 3; CDK5 regulatory subunit associated protein IC53-2; ischemic heart CDK5 activator-binding protein C53; LXXLL/leucine-zipper-containing ARFbinding protein
UniProt Protein Name:	CDK5 regulatory subunit-associated protein 3
UniProt Synonym Protein Names:	CDK5 activator-binding protein C53; Protein HSF-27
Protein Family:	CDK5RAP3-like protein
UniProt Gene Name:	CDK5RAP3
UniProt Entry Name:	CK5P3_HUMAN

Western blot analysis of extracts of various cell lines, using CDK5RAP3 antibody (CAB13128) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

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Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)