KO Validated Antibodies 1

Anti-CDK5 Antibody (CAB18080)[KO Validated]

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SKU:
CAB18080
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Death

Description

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Antibody Name:Anti-CDK5 Antibody [KO Validated]
Antibody SKU:CAB18080
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-292 of human CDK5 (NP_004926.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:10 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:293T
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-292 of human CDK5 (NP_004926.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MQKY EKLE KIGE GTYG TVFK AKNR ETHE IVAL KRVR LDDD DEGV PSSA LREI CLLK ELKH KNIV RLHD VLHS DKKL TLVF EFCD QDLK KYFD SCNG DLDP EIVK SFLF QLLK GLGF CHSR NVLH RDLK PQNL LINR NGEL KLAD FGLA RAFG IPVR CYSA EVVT LWYR PPDV LFGA KLYS TSID MWSA GCIF AELA NAGR PLFP GNDV DDQL KRIF RLLG TPTE EQWP SMTK LPDY KPYP MYPA TTSL VNVV PKLN ATGR DLLQ NLLK CNPV QRIS AEEA LQHP YFSD FCPP
Gene ID:1020
Uniprot:Q00535
Cellular Location:Cell junction, Cell membrane, Cell projection, Cytoplasm, Nucleus, Perikaryon, Peripheral membrane protein, growth cone, lamellipodium, postsynaptic cell membrane, postsynaptic density, synapse
Calculated MW:29kDa/33kDa
Observed MW:30kDa
Anti-CDK5 Antibody [KO Validated] (CAB18080)Western blot analysis of extracts from normal (control) and CDK5 knockout (KO) 293T cells, using CDK5 antibody (CAB18080) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Synonyms:CDK5, LIS7, PSSALRE
Background:This gene encodes a proline-directed serine/threonine kinase that is a member of the cyclin-dependent kinase family of proteins. Unlike other members of the family, the protein encoded by this gene does not directly control cell cycle regulation. Instead the protein, which is predominantly expressed at high levels in mammalian postmitotic central nervous system neurons, functions in diverse processes such as synaptic plasticity and neuronal migration through phosphorylation of proteins required for cytoskeletal organization, endocytosis and exocytosis, and apoptosis. In humans, an allelic variant of the gene that results in undetectable levels of the protein has been associated with lethal autosomal recessive lissencephaly-7. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:CDK5: a protein kinase of the CDK family. Unlike other members of this family, it is not activated by cyclins but by p35 (CDK5R1) and p39. An important regulator of neuronal positioning during brain development. May also play a role in synaptogenesis and neurotransmission. Substrates include TAU, MAP2, NF-H and -M, Nudel, PDE6, beta-catenin, amphyphysin, dynamin I, synapsin 1, Munc-18, and NMDA receptor 2A. Plays a role in myogenesis, haematopoietic cell differentiation, spermatogenesis, insulin secretion, and lens differentiation. Implicated in the pathology of neurofibrillary tangles and formation of senile plaques, hallmarks of Alzheimer?s disease. Induces tau phosphorylation and aggregation and neurofibrillary tangle deposition and neurodegeneration in in vitro and in vivo animal models. Brain samples from Alzeimer?s pateints show elevated CDK5 activity.
UniProt Protein Details:

Protein type:Protein kinase, CMGC; Kinase, protein; Cell cycle regulation; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; CMGC group; CDK family; CDK5 subfamily; CDK/CDK5 subfamily

Chromosomal Location of Human Ortholog: 7q36

Cellular Component: axon; cell junction; cell soma; cytoplasm; cytosol; dendrite; growth cone; membrane; neuromuscular junction; nucleoplasm; nucleus; plasma membrane; postsynaptic density

Molecular Function:acetylcholine receptor activator activity; cyclin-dependent protein kinase activity; ErbB-2 class receptor binding; ErbB-3 class receptor binding; kinase activity; protein binding; protein kinase activity; protein serine/threonine kinase activity; tau-protein kinase activity

Biological Process: axon extension; cell proliferation; negative regulation of proteolysis; negative regulation of transcription, DNA-dependent; neurite development; neuron apoptosis; neuron differentiation; neuron migration; oligodendrocyte differentiation; peptidyl-serine phosphorylation; phosphorylation; positive regulation of neuron apoptosis; regulation of apoptosis; regulation of macroautophagy; regulation of synaptic plasticity; synaptic transmission; synaptic vesicle endocytosis; synaptic vesicle exocytosis; synaptogenesis

Disease: Lissencephaly 7 With Cerebellar Hypoplasia

NCBI Summary:This gene encodes a proline-directed serine/threonine kinase that is a member of the cyclin-dependent kinase family of proteins. Unlike other members of the family, the protein encoded by this gene does not directly control cell cycle regulation. Instead the protein, which is predominantly expressed at high levels in mammalian postmitotic central nervous system neurons, functions in diverse processes such as synaptic plasticity and neuronal migration through phosphorylation of proteins required for cytoskeletal organization, endocytosis and exocytosis, and apoptosis. In humans, an allelic variant of the gene that results in undetectable levels of the protein has been associated with lethal autosomal recessive lissencephaly-7. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2015]
UniProt Code:Q00535
NCBI GenInfo Identifier:4033704
NCBI Gene ID:1020
NCBI Accession:Q00535.3
UniProt Secondary Accession:Q00535,A1XKG3,
UniProt Related Accession:Q00535
Molecular Weight:29,544 Da
NCBI Full Name:Cyclin-dependent-like kinase 5
NCBI Synonym Full Names:cyclin dependent kinase 5
NCBI Official Symbol:CDK5  
NCBI Official Synonym Symbols:LIS7; PSSALRE  
NCBI Protein Information:cyclin-dependent-like kinase 5
UniProt Protein Name:Cyclin-dependent-like kinase 5
UniProt Synonym Protein Names:Cell division protein kinase 5; Serine/threonine-protein kinase PSSALRE; Tau protein kinase II catalytic subunit; TPKII catalytic subunit
Protein Family:CDK5RAP1-like protein
UniProt Gene Name:CDK5  
UniProt Entry Name:CDK5_HUMAN
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