Name: Anti-CDK2AP1 Antibody (CAB19985)[KO Validated]
Brand: KO Validated Antibodies 2
SKU: CAB19985
Availability: OutOfStock

Antibody Name:	Anti-CDK2AP1 Antibody [KO Validated]
Antibody SKU:	CAB19985
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant protein of human CDK2AP1.

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human
Positive Samples:	293T

Immunogen:	Recombinant protein of human CDK2AP1.
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	8099
Uniprot:	O14519
Cellular Location:
Calculated MW:	9kDa/12kDa
Observed MW:	12KDa

Anti-CDK2AP1 Antibody [KO Validated] (CAB19985)

Western blot analysis of extracts from normal (control) and CDK2AP1 knockout (KO) 293T cells, using CDK2AP1 antibody (CAB19985) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.

Synonyms:

CDK2AP1, DOC1, DORC1, ST19, doc-1, p12DOC-1

Background:

The protein encoded by this gene is a cyclin-dependent kinase 2 (CDK2) -associated protein which is thought to negatively regulate CDK2 activity by sequestering monomeric CDK2, and targeting CDK2 for proteolysis. This protein was found to also interact with DNA polymerase alpha/primase and mediate the phosphorylation of the large p180 subunit, which suggests a regulatory role in DNA replication during the S-phase of the cell cycle. This protein also forms a core subunit of the nucleosome remodeling and histone deacetylation (NURD) complex that epigenetically regulates embryonic stem cell differentiation. This gene thus plays a role in both cell-cycle and epigenetic regulation. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

UniProt Protein Function:	CDK2AP1: specific inhibitor of the cell-cycle kinase CDK2. Belongs to the CDK2AP family.
UniProt Protein Details:	Protein type:DNA replication; Tumor suppressor; Cell cycle regulation Chromosomal Location of Human Ortholog: 12q24.31 Cellular Component: perinuclear region of cytoplasm; nucleus Biological Process: positive regulation of protein amino acid phosphorylation; DNA-dependent DNA replication; cell cycle
NCBI Summary:	The protein encoded by this gene is a cyclin-dependent kinase 2 (CDK2) -associated protein which is thought to negatively regulate CDK2 activity by sequestering monomeric CDK2, and targeting CDK2 for proteolysis. This protein was found to also interact with DNA polymerase alpha/primase and mediate the phosphorylation of the large p180 subunit, which suggests a regulatory role in DNA replication during the S-phase of the cell cycle. This protein also forms a core subunit of the nucleosome remodeling and histone deacetylation (NURD) complex that epigenetically regulates embryonic stem cell differentiation. This gene thus plays a role in both cell-cycle and epigenetic regulation. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2012]
UniProt Code:	O14519
NCBI GenInfo Identifier:	6014988
NCBI Gene ID:	8099
NCBI Accession:	O14519.1
UniProt Secondary Accession:	O14519,F5GYA4,
UniProt Related Accession:	O14519
Molecular Weight:	115
NCBI Full Name:	Cyclin-dependent kinase 2-associated protein 1
NCBI Synonym Full Names:	cyclin-dependent kinase 2 associated protein 1
NCBI Official Symbol:	CDK2AP1
NCBI Official Synonym Symbols:	DOC1; ST19; DORC1; doc-1; p12DOC-1
NCBI Protein Information:	cyclin-dependent kinase 2-associated protein 1; Deleted in oral cancer-1; deleted in oral cancer 1; CDK2-associated protein 1; putative oral cancer suppressor
UniProt Protein Name:	Cyclin-dependent kinase 2-associated protein 1
UniProt Synonym Protein Names:	Deleted in oral cancer 1; DOC-1; Putative oral cancer suppressor
Protein Family:	Cyclin-dependent kinase 2-associated protein
UniProt Gene Name:	CDK2AP1
UniProt Entry Name:	CDKA1_HUMAN