Epigenetics & Nuclear Signaling Antibodies 2

Anti-CDK12 Antibody (CAB14894)

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SKU:
CAB14894
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-CDK12 Antibody
Antibody SKU:CAB14894
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 80-290 of human CDK12 (NP_057591.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, HepG2, Mouse liver, Rat liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 80-290 of human CDK12 (NP_057591.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:SDTF SDDM AFKL DRRE NDER RGSD RSDR LHKH RHHQ HRRS RDLL KAKQ TEKE KSQE VSSK SGSM KDRI SGSS KRSN EETD DYGK AQVA KSSS KESR SSKL HKEK TRKE RELK SGHK DRSK SHRK RETP KSYK TVDS PKRR SRSP HRKW SDSS KQDD SPSG ASYG QDYD LSPS RSHT SSNY DSYK KSPG STSR RQSV SPPY KEPS AYQS STR
Gene ID:51755
Uniprot:Q9NYV4
Cellular Location:Nucleus, Nucleus speckle
Calculated MW:141kDa/163kDa/164kDa
Observed MW:164kDa
Synonyms:CDK12, CRK7, CRKR, CRKRS
Background:
UniProt Protein Function:CDK12: a Ser/Thr protein kinase of the CMGC group and CDK family. Phosphorylates the C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit RPB1, thereby acting as a key regulator of transcription elongation. Required for RNA splicing, possibly by phosphorylating SRSF1/SF2. Interacts with CCNL1 and CCNL2. Colocalized with nuclear speckles throughout interphase. Has a highly phosphorylated arginine/serine-rich (RS) domain N-terminal to the kinase domain. Chromosomal aberrations involving CDK12 may be a cause gastric cancer. Deletions within 17q12 region producing fusion transcripts with ERBB2, leading to CDK12-ERBB2 fusion leading to trunctated CDK12 protein not in-frame with ERBB2. Three isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:RNA splicing; Protein kinase, CMGC; Motility/polarity/chemotaxis; Kinase, protein; Spliceosome; EC 2.7.11.23; Cell cycle regulation; EC 2.7.11.22; Protein kinase, Ser/Thr (non-receptor); CMGC group; CDK family; CRK7 subfamily; CDK/CRK7 subfamily

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: nucleoplasm; nuclear cyclin-dependent protein kinase holoenzyme complex; nucleolus; nuclear speck; nucleus

Molecular Function:RNA polymerase subunit kinase activity; protein binding; cyclin binding; cyclin-dependent protein kinase activity; ATP binding; protein kinase activity

Biological Process: regulation of cell cycle; protein amino acid autophosphorylation; regulation of MAP kinase activity; RNA splicing; mRNA processing

UniProt Code:Q9NYV4
NCBI GenInfo Identifier:308153421
NCBI Gene ID:51755
NCBI Accession:Q9NYV4.2
UniProt Secondary Accession:Q9NYV4,O94978, A7E2B2, B4DYX4, B9EIQ6,
UniProt Related Accession:Q9NYV4
Molecular Weight:141,449 Da
NCBI Full Name:Cyclin-dependent kinase 12
NCBI Synonym Full Names:cyclin-dependent kinase 12
NCBI Official Symbol:CDK12  
NCBI Official Synonym Symbols:CRK7; CRKR; CRKRS  
NCBI Protein Information:cyclin-dependent kinase 12; CDC2-related protein kinase 7; cell division protein kinase 12; Cdc2-related kinase, arginine/serine-rich; cell division cycle 2-related protein kinase 7
UniProt Protein Name:Cyclin-dependent kinase 12
UniProt Synonym Protein Names:Cdc2-related kinase, arginine/serine-rich; CrkRS; Cell division cycle 2-related protein kinase 7; CDC2-related protein kinase 7; Cell division protein kinase 12; hCDK12
UniProt Gene Name:CDK12  
UniProt Entry Name:CDK12_HUMAN
Anti-CDK12 Antibody (CAB14894)Western blot analysis of extracts of various cell lines, using CDK12 antibody (CAB14894) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
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