Antibody Name:	Anti-CDK10 Antibody
Antibody SKU:	CAB2690
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-283 of human CDK10 (NP_001092003.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	293T, HT-29

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-283 of human CDK10 (NP_001092003.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MDKE KDGI PISS LREI TLLL RLRH PNIV ELKE VVVG NHLE SIFL VMGY CEQD LASL LENM PTPF SEAQ VKCI VLQV LRGL QYLH RNFI IHRD LKVS NLLM TDKG CVKT ADFG LARA YGVP VKPM TPKV VTLW YRAP ELLL GTTT QTTS IDMW AVGC ILAE LLAH RPLL PGTS EIHQ IDLI VQLL GTPS ENIW PGFS KLPL VGQY SLRK QPYN NLKH KFPW LSEA GLRL LHFL FMAT AGDC LESS YFKE KPLP CEPE LMPT FPHH RNKR AAPA TSEG QSKR CKP
Gene ID:	8558
Uniprot:	Q15131
Cellular Location:
Calculated MW:	17kDa/21kDa/30kDa/31kDa/32kDa/41kDa
Observed MW:	46kDa

Synonyms:

CDK10, PISSLRE

Background:

The protein encoded by this gene belongs to the CDK subfamily of the Ser/Thr protein kinase family. The CDK subfamily members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and are known to be essential for cell cycle progression. This kinase has been shown to play a role in cellular proliferation and its function is limited to cell cycle G2-M phase. Multiple transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	CDK10: a CMGC kinase of the CDK family. Plays a role in cellular proliferation. Its function is limited to cell cycle G2-M phase. At least three alternatively spliced isoforms have been reported, two of which contain multiple non-AUG translation initiation sites.
UniProt Protein Details:	Protein type:Protein kinase, Ser/Thr (non-receptor); Protein kinase, CMGC; Cell cycle regulation; Kinase, protein; EC 2.7.11.22; CMGC group; CDK family; CDK/CDK10 subfamily; CDK10 subfamily Chromosomal Location of Human Ortholog: 16q24 Molecular Function:protein binding; cyclin-dependent protein kinase activity; ATP binding Biological Process: negative regulation of cell proliferation; traversing start control point of mitotic cell cycle; positive regulation of MAPKKK cascade; protein amino acid phosphorylation
NCBI Summary:	The protein encoded by this gene belongs to the CDK subfamily of the Ser/Thr protein kinase family. The CDK subfamily members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and are known to be essential for cell cycle progression. This kinase has been shown to play a role in cellular proliferation and its function is limited to cell cycle G2-M phase. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]
UniProt Code:	Q15131
NCBI GenInfo Identifier:	6226784
NCBI Gene ID:	8558
NCBI Accession:	Q15131.1
UniProt Secondary Accession:	Q15131,Q0VGZ7, Q15130, Q6PJC0, A8K370, A8K8I6, A8MXU6 B3KQJ3, B7Z420, D3DX82, D3DX83,
UniProt Related Accession:	Q15131
Molecular Weight:	272
NCBI Full Name:	Cyclin-dependent kinase 10
NCBI Synonym Full Names:	cyclin-dependent kinase 10
NCBI Official Symbol:	CDK10
NCBI Official Synonym Symbols:	PISSLRE
NCBI Protein Information:	cyclin-dependent kinase 10; CDC2-related protein kinase; cell division protein kinase 10; cyclin-dependent kinase (CDC2-like) 10; cyclin-dependent kinase related protein; serine/threonine protein kinase PISSLRE; serine/threonine-protein kinase PISSLRE
UniProt Protein Name:	Cyclin-dependent kinase 10
UniProt Synonym Protein Names:	Cell division protein kinase 10; Serine/threonine-protein kinase PISSLRE
Protein Family:	Cyclin-dependent kinase
UniProt Gene Name:	CDK10
UniProt Entry Name:	CDK10_HUMAN

	Western blot analysis of extracts of various cell lines, using CDK10 antibody (CAB2690) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded mouse brain using CDK10 antibody (CAB2690) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U2OS cells using CDK10 antibody (CAB2690) at dilution of 1:100. Blue: DAPI for nuclear staining.