Cell Biology Antibodies 4

Anti-CDH8 Antibody (CAB14236)

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SKU:
CAB14236
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-CDH8 Antibody
Antibody SKU:CAB14236
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 500-610 of human CDH8 (NP_001787.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:U-87MG, U-251MG, Mouse brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 500-610 of human CDH8 (NP_001787.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:YEAF LCEN GKPG QVIQ TVSA MDKD DPKN GHYF LYSL LPEM VNNP NFTI KKNE DNSL SILA KHNG FNRQ KQEV YLLP IIIS DSGN PPLS STST LTIR VCGC SNDG VVQS CNV
Gene ID:1006
Uniprot:P55286
Cellular Location:Cell membrane, Single-pass type I membrane protein
Calculated MW:88kDa
Observed MW:110kDa
Synonyms:CDH8, Nbla04261
Background:This gene encodes a type II classical cadherin from the cadherin superfamily, integral membrane proteins that mediate calcium-dependent cell-cell adhesion. Mature cadherin proteins are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain. The extracellular domain consists of 5 subdomains, each containing a cadherin motif, and appears to determine the specificity of the protein's homophilic cell adhesion activity. Type II (atypical) cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins. This particular cadherin is expressed in brain and is putatively involved in synaptic adhesion, axon outgrowth and guidance.
UniProt Protein Function:CDH8: Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.
UniProt Protein Details:

Protein type:Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: plasma membrane

Biological Process: cell adhesion

NCBI Summary:This gene encodes a type II classical cadherin from the cadherin superfamily, integral membrane proteins that mediate calcium-dependent cell-cell adhesion. Mature cadherin proteins are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain. The extracellular domain consists of 5 subdomains, each containing a cadherin motif, and appears to determine the specificity of the protein's homophilic cell adhesion activity. Type II (atypical) cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins. This particular cadherin is expressed in brain and is putatively involved in synaptic adhesion, axon outgrowth and guidance. [provided by RefSeq, Jul 2008]
UniProt Code:P55286
NCBI GenInfo Identifier:13432121
NCBI Gene ID:1006
NCBI Accession:P55286.2
UniProt Secondary Accession:P55286,Q14DC6, Q9ULB2, B3KWC1,
UniProt Related Accession:P55286
Molecular Weight:88,253 Da
NCBI Full Name:Cadherin-8
NCBI Synonym Full Names:cadherin 8
NCBI Official Symbol:CDH8  
NCBI Official Synonym Symbols:Nbla04261  
NCBI Protein Information:cadherin-8
UniProt Protein Name:Cadherin-8
Protein Family:Cadherin
UniProt Gene Name:CDH8  
UniProt Entry Name:CADH8_HUMAN
Anti-CDH8 Antibody (CAB14236)Western blot analysis of extracts of various cell lines, using CDH8 antibody (CAB14236) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
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