Antibody Name:	Anti-CDH16 Antibody
Antibody SKU:	CAB7882
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 19-230 of human CDH16 (NP_004053.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	A-549, 293T, HepG2, Mouse kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 19-230 of human CDH16 (NP_004053.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	KAQP AELS VEVP ENYG GNFP LYLT KLPL PREG AEGQ IVLS GDSG KATE GPFA MDPD SGFL LVTR ALDR EEQA EYQL QVTL EMQD GHVL WGPQ PVLV HVKD ENDQ VPHF SQAI YRAR LSRG TRPG IPFL FLEA SDRD EPGT ANSD LRFH ILSQ APAQ PSPD MFQL EPRL GALA LSPK GSTS LDHA LERT YQLL VQVK DMGD QASG HQAT ATVE
Gene ID:	1014
Uniprot:	O75309
Cellular Location:	Cell membrane, Single-pass type I membrane protein
Calculated MW:	79kDa/85kDa/87kDa/89kDa
Observed MW:	110kDa

Synonyms:

CDH16

Background:

This gene is a member of the cadherin superfamily, genes encoding calcium-dependent, membrane-associated glycoproteins. Mapped to a previously identified cluster of cadherin genes on chromosome 16q22.1, the gene localizes with superfamily members CDH1, CDH3, CDH5, CDH8 and CDH11. The protein consists of an extracellular domain containing 6 cadherin domains, a transmembrane region and a truncated cytoplasmic domain but lacks the prosequence and tripeptide HAV adhesion recognition sequence typical of most classical cadherins. Expression is exclusively in kidney, where the protein functions as the principal mediator of homotypic cellular recognition, playing a role in the morphogenic direction of tissue development. Alternatively spliced transcript variants encoding distinct isoforms have been identified.

UniProt Protein Function:	CDH16: Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Membrane protein, integral Chromosomal Location of Human Ortholog: 16q22.1 Biological Process: cell adhesion
NCBI Summary:	This gene is a member of the cadherin superfamily, genes encoding calcium-dependent, membrane-associated glycoproteins. Mapped to a previously identified cluster of cadherin genes on chromosome 16q22.1, the gene localizes with superfamily members CDH1, CDH3, CDH5, CDH8 and CDH11. The protein consists of an extracellular domain containing 6 cadherin domains, a transmembrane region and a truncated cytoplasmic domain but lacks the prosequence and tripeptide HAV adhesion recognition sequence typical of most classical cadherins. Expression is exclusively in kidney, where the protein functions as the principal mediator of homotypic cellular recognition, playing a role in the morphogenic direction of tissue development. Alternatively spliced transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Mar 2011]
UniProt Code:	O75309
NCBI GenInfo Identifier:	13431340
NCBI Gene ID:	1014
NCBI Accession:	O75309.1
UniProt Secondary Accession:	O75309,Q6UW93, B4DPA8, H3BPD3,
UniProt Related Accession:	O75309
Molecular Weight:	85,774 Da
NCBI Full Name:	Cadherin-16
NCBI Synonym Full Names:	cadherin 16
NCBI Official Symbol:	CDH16
NCBI Protein Information:	cadherin-16
UniProt Protein Name:	Cadherin-16
UniProt Synonym Protein Names:	Kidney-specific cadherin; Ksp-cadherin
Protein Family:	Cadherin
UniProt Gene Name:	CDH16
UniProt Entry Name:	CAD16_HUMAN

	Western blot analysis of extracts of various cell lines, using CDH16 antibody (CAB7882) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat kidney using CDH16 antibody (CAB7882) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse kidney using CDH16 antibody (CAB7882) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of rat kidney using CDH16 Polyclonal Antibody (CAB7882) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.