Cell Biology Antibodies 1

Anti-CDH12 Antibody (CAB10206)

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SKU:
CAB10206
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-CDH12 Antibody
Antibody SKU:CAB10206
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 390-540 of human CDH12 (NP_004052.2).
Application:WB
Recommended Dilution:WB 1:1000 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:U-251MG, HepG2, 22Rv1, Mouse brain, Mouse liver, Rat brain, Rat pancreas, Rat liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 390-540 of human CDH12 (NP_004052.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:TMEV YEDT PVGT IIGA VTAQ DLDV GSSA VRYF IDWK SDGD SYFT IDGN EGTI ATNE LLDR ESTA QYNF SIIA SKVS NPLL TSKV NILI NVLD VNEF PPEI SVPY ETAV CENA KPGQ IIQI VSAA DRDL SPAG QQFS FRLS PEAA IKPN FTV
Gene ID:1010
Uniprot:P55289
Cellular Location:Cell membrane, Single-pass type I membrane protein
Calculated MW:84kDa/88kDa
Observed MW:110kDa
Synonyms:CDH12, CDHB
Background:This gene encodes a type II classical cadherin of the cadherin superfamily. Alternative splicing of this gene results in multiple transcript variants. At least one of these variants encodes a preproprotein that is proteolytically processed to generate the mature cadherin protein. These integral membrane proteins mediate calcium-dependent cell-cell adhesion and are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain. Type II (atypical) cadherins are defined based on their lack of a histidine-alanine-valine (HAV) cell adhesion recognition sequence specific to type I cadherins. This particular cadherin appears to be expressed specifically in the brain and its temporal pattern of expression would be consistent with a role during a critical period of neuronal development, perhaps specifically during synaptogenesis.
UniProt Protein Function:CDH12: Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.
UniProt Protein Details:

Protein type:Membrane protein, integral; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 5p14.3

Cellular Component: integral to membrane; plasma membrane

Molecular Function:calcium ion binding

Biological Process: intercellular junction assembly and maintenance; homophilic cell adhesion

NCBI Summary:This gene encodes a type II classical cadherin from the cadherin superfamily of integral membrane proteins that mediate calcium-dependent cell-cell adhesion. Mature cadherin proteins are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain. Type II (atypical) cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins. This particular cadherin appears to be expressed specifically in the brain and its temporal pattern of expression would be consistent with a role during a critical period of neuronal development, perhaps specifically during synaptogenesis. [provided by RefSeq, Jul 2008]
UniProt Code:P55289
NCBI GenInfo Identifier:158937438
NCBI Gene ID:1010
NCBI Accession:P55289.2
UniProt Secondary Accession:P55289,Q86UD2, B2RBT1,
UniProt Related Accession:P55289
Molecular Weight:88,332 Da
NCBI Full Name:Cadherin-12
NCBI Synonym Full Names:cadherin 12, type 2 (N-cadherin 2)
NCBI Official Symbol:CDH12  
NCBI Official Synonym Symbols:CDHB  
NCBI Protein Information:cadherin-12; Br-cadherin; N-cadherin 2; brain cadherin; brain-cadherin; neuronal cadherin 2; neural type cadherin 2; cadherin, neural type, 2
UniProt Protein Name:Cadherin-12
UniProt Synonym Protein Names:Brain cadherin; BR-cadherin; Neural type cadherin 2; N-cadherin 2
Protein Family:Cadherin
UniProt Gene Name:CDH12  
UniProt Entry Name:CAD12_HUMAN
Anti-CDH12 Antibody (CAB10206)Western blot analysis of extracts of various cell lines, using CDH12 antibody (CAB10206) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
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