Cell Cycle Antibodies 1
Anti-CDCA8 Antibody (CAB15463)
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- SKU:
- CAB15463
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Cycle
Description
| Antibody Name: | Anti-CDCA8 Antibody |
| Antibody SKU: | CAB15463 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-124 of human CDCA8 (NP_060571.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:200 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | A-549, 293T, Mouse testis, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-124 of human CDCA8 (NP_060571.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAPR KGSS RVAK TNSL RRRK LASF LKDF DREV EIRI KQIE SDRQ NLLK EVDN LYNI EILR LPKA LREM NWLD YFAL GGNK QALE EAAT ADLD ITEI NKLT AEAI QTPL KSAK TRKV IQVD EMIV |
| Gene ID: | 55143 |
| Uniprot: | Q53HL2 |
| Cellular Location: | Chromosome, Cytoplasm, Nucleus, centromere, cytoskeleton, nucleolus, spindle |
| Calculated MW: | 31kDa |
| Observed MW: | 36kDa |
| Synonyms: | CDCA8, BOR, BOREALIN, DasraB, MESRGP, borealin |
| Background: | This gene encodes a component of the chromosomal passenger complex. This complex is an essential regulator of mitosis and cell division. This protein is cell-cycle regulated and is required for chromatin-induced microtubule stabilization and spindle formation. Alternate splicing results in multiple transcript variants. Pseudgenes of this gene are found on chromosomes 7, 8 and 16. |
| UniProt Protein Function: | Borealin: Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. In the complex, it may be required to direct the CPC to centromeric DNA. Major effector of the TTK kinase in the control of attachment-error-correction and chromosome alignment. Belongs to the borealin family. |
| UniProt Protein Details: | Protein type:Cell cycle regulation; Nucleolus Chromosomal Location of Human Ortholog: 1p34.3 Cellular Component: intercellular bridge; protein complex; nucleolus; spindle; midbody; nucleus; cytosol; chromosome, pericentric region; chromocenter Molecular Function:protein binding Biological Process: cell division; mitotic cell cycle; mitotic metaphase plate congression; chromosome organization and biogenesis |
| NCBI Summary: | This gene encodes a component of the chromosomal passenger complex. This complex is an essential regulator of mitosis and cell division. This protein is cell-cycle regulated and is required for chromatin-induced microtubule stabilization and spindle formation. Alternate splicing results in multiple transcript variants. Pseudgenes of this gene are found on chromosomes 7, 8 and 16. [provided by RefSeq, Apr 2013] |
| UniProt Code: | Q53HL2 |
| NCBI GenInfo Identifier: | 110832774 |
| NCBI Gene ID: | 55143 |
| NCBI Accession: | Q53HL2.2 |
| UniProt Secondary Accession: | Q53HL2,Q53HN1, Q96AM3, Q9NVW5, D3DPT4, |
| UniProt Related Accession: | Q53HL2 |
| Molecular Weight: | 280 |
| NCBI Full Name: | Borealin |
| NCBI Synonym Full Names: | cell division cycle associated 8 |
| NCBI Official Symbol: | CDCA8 |
| NCBI Official Synonym Symbols: | BOR; DasraB; MESRGP; BOREALIN |
| NCBI Protein Information: | borealin; Dasra B; dasra-B; hDasra-B; cell division cycle-associated protein 8; pluripotent embryonic stem cell-related gene 3 protein |
| UniProt Protein Name: | Borealin |
| UniProt Synonym Protein Names: | Cell division cycle-associated protein 8; Dasra-B; hDasra-B; Pluripotent embryonic stem cell-related gene 3 protein |
| UniProt Gene Name: | CDCA8 |
| UniProt Entry Name: | BOREA_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CDCA8 antibody (CAB15463) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
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