Cell Cycle Antibodies 1
Anti-CDCA3 Antibody (CAB16151)
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- SKU:
- CAB16151
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Cycle
Description
| Antibody Name: | Anti-CDCA3 Antibody |
| Antibody SKU: | CAB16151 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 100 to the C-terminus of human CDCA3 (NP_001284531.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | HeLa, Mouse lung |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 100 to the C-terminus of human CDCA3 (NP_001284531.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | PLGT QLSV EEQM PPWN QTEF PSKQ VFSK EEAR QPTE TPVA SQSS DKPS RDPE TPRS SGSM RNRW KPNS SKVL GRSP LTIL QDDN SPGT LTLR QGKR PSPL SENV SELK EGAI LGTG RLLK TGGR AWEQ GQDH DKEN QHFP LVES |
| Gene ID: | 83461 |
| Uniprot: | Q99618 |
| Cellular Location: | Cytoplasm, cytosol |
| Calculated MW: | 28kDa |
| Observed MW: | 29kDa |
| Synonyms: | CDCA3, GRCC8, TOME-1 |
| Background: |
| UniProt Protein Function: | CDCA3: F-box-like protein which is required for entry into mitosis. Acts by participating in E3 ligase complexes that mediate the ubiquitination and degradation of WEE1 kinase at G2/M phase. |
| UniProt Protein Details: | Protein type:Cell cycle regulation Chromosomal Location of Human Ortholog: 12p13 Molecular Function:protein binding |
| UniProt Code: | Q99618 |
| NCBI GenInfo Identifier: | 74732787 |
| NCBI Gene ID: | 83461 |
| NCBI Accession: | Q99618.1 |
| UniProt Secondary Accession: | Q99618,A8K5V6, D3DUS6, |
| UniProt Related Accession: | Q99618 |
| Molecular Weight: | 28,998 Da |
| NCBI Full Name: | Cell division cycle-associated protein 3 |
| NCBI Synonym Full Names: | cell division cycle associated 3 |
| NCBI Official Symbol: | CDCA3 |
| NCBI Official Synonym Symbols: | GRCC8; TOME-1 |
| NCBI Protein Information: | cell division cycle-associated protein 3 |
| UniProt Protein Name: | Cell division cycle-associated protein 3 |
| UniProt Synonym Protein Names: | Gene-rich cluster protein C8; Trigger of mitotic entry protein 1; TOME-1 |
| Protein Family: | Cell division cycle-associated protein |
| UniProt Gene Name: | CDCA3 |
| UniProt Entry Name: | CDCA3_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CDCA3 antibody (CAB16151) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 2min. |
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