Cell Cycle Antibodies 2

Anti-CDCA2 Antibody (CAB9291)

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SKU:
CAB9291
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Cycle

Description

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Antibody Name:Anti-CDCA2 Antibody
Antibody SKU:CAB9291
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 724-1023 of human CDCA2 (NP_689775.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:HeLa, Jurkat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 724-1023 of human CDCA2 (NP_689775.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:SDSP KPAL TLQQ GQEF SAGG QNAE NLCQ FFKI SPDL NIKC ERKD DFLG AAEG KLQC NRLM PNSQ KDCH CLGD VLIE NTKE SKSQ SEDL GRKP MESS SVVS CRDR KDRR RSMC YSDG RSLH LEKN GNHT PSSS VGSS VEIS LENS ELFK DLSD AIEQ TFQR RNSE TKVR RSTR LQKD LENE GLVW ISLP LPST SQKA KRRT ICTF DSSG FESM SPIK ETVS SRQK PQMA PPVS DPEN SQGP AAGS SDEP GKRR KSFC ISTL ANTK ATSQ FKGY RRRS SLNG KGES SLTA LERI EHNG ERKQ
Gene ID:157313
Uniprot:Q69YH5
Cellular Location:Nucleus
Calculated MW:7kDa/112kDa
Observed MW:130kDa
Synonyms:CDCA2, PPP1R81, Repo-Man
Background:This gene encodes a targeting subunit of the cell-cycle associated protein, protein phosphatase 1, with a role in targeting this protein to chromatin during anaphase. These two proteins comprise a phosphatase complex that is involved in nuclear envelope reformation and regulation of the DNA damage response. The encoded protein may also play a role in cancer progression. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:Regulator of chromosome structure during mitosis required for condensin-depleted chromosomes to retain their compact architecture through anaphase. Acts by mediating the recruitment of phopsphatase PP1-gamma subunit (PPP1CC) to chromatin at anaphase and into the following interphase. At anaphase onset, its association with chromatin targets a pool of PPP1CC to dephosphorylate substrates.
NCBI Summary:This gene encodes a targeting subunit of the cell-cycle associated protein, protein phosphatase 1, with a role in targeting this protein to chromatin during anaphase. These two proteins comprise a phosphatase complex that is involved in nuclear envelope reformation and regulation of the DNA damage response. The encoded protein may also play a role in cancer progression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]
UniProt Code:Q69YH5
NCBI GenInfo Identifier:961658079
NCBI Gene ID:157313
NCBI Accession:NP_001304835.1
UniProt Secondary Accession:Q69YH5,Q3SX74, Q4G0W0, Q5RKN0, Q69YI4, Q6P464, Q8N7C1
UniProt Related Accession:Q69YH5
Molecular Weight:113 KD
NCBI Full Name:cell division cycle-associated protein 2 isoform 2
NCBI Synonym Full Names:cell division cycle associated 2
NCBI Official Symbol:CDCA2  
NCBI Official Synonym Symbols:PPP1R81; Repo-Man  
NCBI Protein Information:cell division cycle-associated protein 2
UniProt Protein Name:Cell division cycle-associated protein 2
UniProt Synonym Protein Names:Recruits PP1 onto mitotic chromatin at anaphase protein; Repo-Man
Protein Family:Cell division cycle-associated protein
UniProt Gene Name:CDCA2  
Anti-CDCA2 Polyclonal Antibody (CAB9291)Western blot analysis of extracts of various cell lines, using CDCA2 antibody (CAB9291) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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