Cell Cycle Antibodies 1
Anti-CDC34 Antibody (CAB5457)
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- SKU:
- CAB5457
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Cycle
Description
| Antibody Name: | Anti-CDC34 Antibody |
| Antibody SKU: | CAB5457 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-236 of human CDC34 (NP_004350.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, Jurkat, C6, SH-SY5Y, Mouse testis |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-236 of human CDC34 (NP_004350.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MARP LVPS SQKA LLLE LKGL QEEP VEGF RVTL VDEG DLYN WEVA IFGP PNTY YEGG YFKA RLKF PIDY PYSP PAFR FLTK MWHP NIYE TGDV CISI LHPP VDDP QSGE LPSE RWNP TQNV RTIL LSVI SLLN EPNT FSPA NVDA SVMY RKWK ESKG KDRE YTDI IRKQ VLGT KVDA ERDG VKVP TTLA EYCV KTKA PAPD EGSD LFYD DYYE DGEV EEEA DSCF GDDE DDSG TEES |
| Gene ID: | 997 |
| Uniprot: | P49427 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 26kDa |
| Observed MW: | 32KDa |
| Synonyms: | CDC34, E2-CDC34, UBC3, UBCH3, UBE2R1 |
| Background: | The protein encoded by this gene is a member of the ubiquitin-conjugating enzyme family. Ubiquitin-conjugating enzyme catalyzes the covalent attachment of ubiquitin to other proteins. This protein is a part of the large multiprotein complex, which is required for ubiquitin-mediated degradation of cell cycle G1 regulators, and for the initiation of DNA replication. |
| UniProt Protein Function: | CDC34: a member of the ubiquitin-conjugating enzyme family. Is a part of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex which is required for ubiquitin-mediated degradation of cell cycle G1 regulators. Required for the initiation of DNA replication. |
| UniProt Protein Details: | Protein type:Ligase; Ubiquitin ligase; EC 6.3.2.19; Ubiquitin conjugating system Chromosomal Location of Human Ortholog: 19p13.3 Cellular Component: cytoplasm; nucleoplasm; nucleus Molecular Function:ATP binding; protein binding; ubiquitin protein ligase binding; ubiquitin-protein ligase activity Biological Process: DNA replication initiation; G1/S transition of mitotic cell cycle; positive regulation of neuron apoptosis; proteasomal ubiquitin-dependent protein catabolic process; protein modification process; protein polyubiquitination; protein ubiquitination |
| NCBI Summary: | The protein encoded by this gene is a member of the ubiquitin-conjugating enzyme family. Ubiquitin-conjugating enzyme catalyzes the covalent attachment of ubiquitin to other proteins. This protein is a part of the large multiprotein complex, which is required for ubiquitin-mediated degradation of cell cycle G1 regulators, and for the initiation of DNA replication. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P49427 |
| NCBI GenInfo Identifier: | 2507505 |
| NCBI Gene ID: | 997 |
| NCBI Accession: | P49427.2 |
| UniProt Secondary Accession: | P49427,A8K689, |
| UniProt Related Accession: | P49427 |
| Molecular Weight: | 26,737 Da |
| NCBI Full Name: | Ubiquitin-conjugating enzyme E2 R1 |
| NCBI Synonym Full Names: | cell division cycle 34 |
| NCBI Official Symbol: | CDC34 |
| NCBI Official Synonym Symbols: | UBC3; UBCH3; UBE2R1; E2-CDC34 |
| NCBI Protein Information: | ubiquitin-conjugating enzyme E2 R1 |
| UniProt Protein Name: | Ubiquitin-conjugating enzyme E2 R1 |
| UniProt Synonym Protein Names: | (E3-independent) E2 ubiquitin-conjugating enzyme R1 (EC:2.3.2.24 |
| Protein Family: | Ubiquitin-conjugating enzyme |
| UniProt Gene Name: | CDC34 |
| UniProt Entry Name: | UB2R1_HUMAN |
 | Western blot analysis of extracts of Mouse testis, using CDC34 antibody (CAB5457) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Western blot analysis of extracts of various cell lines, using CDC34 antibody (CAB5457) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunofluorescence analysis of HeLa cells using CDC34 antibody (CAB5457). |
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