Signal Transduction Antibodies 2

Anti-CDC27 Antibody (CAB1954)

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SKU:
CAB1954
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Signal Transduction

Description

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Antibody Name:Anti-CDC27 Antibody
Antibody SKU:CAB1954
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 551-830 of human CDC27 (NP_001107563.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 551-830 of human CDC27 (NP_001107563.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:HLQK DVAL SVLS KDLT DMDK NSPE AWCA AGNC FSLQ REHD IAIK FFQR AIQV DPNY AYAY TLLG HEFV LTEE LDKA LACF RNAI RVNP RHYN AWYG LGMI YYKQ EKFS LAEM HFQK ALDI NPQS SVLL CHIG VVQH ALKK SEKA LDTL NKAI VIDP KNPL CKFH RASV LFAN EKYK SALQ ELEE LKQI VPKE SLVY FLIG KVYK KLGQ THLA LMNF SWAM DLDP KGAN NQIK EAID KRYL PDDE EPIT QEEQ IMGT DESQ ESSM TDAD DTQL HAAE SDEF
Gene ID:996
Uniprot:P30260
Cellular Location:Nucleus
Calculated MW:91kDa/92kDa
Observed MW:110kDa
Synonyms:CDC27, ANAPC3, APC3, CDC27Hs, D0S1430E, D17S978E, H-NUC, HNUC, NUC2
Background:The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of the anaphase-promoting complex (APC), which is composed of eight protein subunits and is highly conserved in eukaryotic cells. This complex catalyzes the formation of cyclin B-ubiquitin conjugate, which is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. The protein encoded by this gene and three other members of the APC complex contain tetratricopeptide (TPR) repeats, which are important for protein-protein interactions. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and it may thus be involved in controlling the timing of mitosis. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 2, 22 and Y.
UniProt Protein Function:CDC27: Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. The APC/C is composed of at least 12 subunits. Interacts with RB. Interacts with FAM168B/MANI. Belongs to the APC3/CDC27 family.
UniProt Protein Details:

Protein type:Cell cycle regulation

Chromosomal Location of Human Ortholog: 17q21.32

Cellular Component: nucleoplasm; centrosome; anaphase-promoting complex; spindle microtubule; cytoplasm; spindle; cytosol; nucleus

Molecular Function:protein binding; protein phosphatase binding

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; cell proliferation; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; mitotic cell cycle spindle assembly checkpoint; mitotic cell cycle; mitotic metaphase/anaphase transition

NCBI Summary:The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of the anaphase-promoting complex (APC), which is composed of eight protein subunits and is highly conserved in eukaryotic cells. This complex catalyzes the formation of cyclin B-ubiquitin conjugate, which is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. The protein encoded by this gene and three other members of the APC complex contain tetratricopeptide (TPR) repeats, which are important for protein-protein interactions. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and it may thus be involved in controlling the timing of mitosis. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 2, 22 and Y. [provided by RefSeq, May 2014]
UniProt Code:P30260
NCBI GenInfo Identifier:12644198
NCBI Gene ID:996
NCBI Accession:P30260.2
UniProt Secondary Accession:P30260,Q16349, Q96F35, G3V1C4,
UniProt Related Accession:P30260
Molecular Weight:824
NCBI Full Name:Cell division cycle protein 27 homolog
NCBI Synonym Full Names:cell division cycle 27
NCBI Official Symbol:CDC27  
NCBI Official Synonym Symbols:APC3; HNUC; NUC2; H-NUC; ANAPC3; CDC27Hs; D0S1430E; D17S978E  
NCBI Protein Information:cell division cycle protein 27 homolog; nuc2 homolog; cell division cycle 27 homolog; anaphase promoting complex subunit 3; anaphase-promoting complex, protein 3
UniProt Protein Name:Cell division cycle protein 27 homolog
UniProt Synonym Protein Names:Anaphase-promoting complex subunit 3; APC3; CDC27 homolog; CDC27Hs; H-NUC
Protein Family:Cell division cycle protein
UniProt Gene Name:CDC27  
UniProt Entry Name:CDC27_HUMAN
Anti-CDC27 Antibody (CAB1954)Western blot analysis of extracts of HeLa cells, using CDC27 antibody (CAB1954) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021).
Anti-CDC27 Antibody (CAB1954)Immunohistochemistry of paraffin-embedded human colon carcinoma using CDC27 antibody (CAB1954) at dilution of 1:200 (40x lens).
Anti-CDC27 Antibody (CAB1954)Immunohistochemistry of paraffin-embedded mouse brain using CDC27 antibody (CAB1954) at dilution of 1:200 (40x lens).
Anti-CDC27 Antibody (CAB1954)Immunohistochemistry of paraffin-embedded mouse heart using CDC27 antibody (CAB1954) at dilution of 1:200 (40x lens).
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