Cell Cycle Antibodies 1

Anti-CDC25B Antibody (CAB0534)

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SKU:
CAB0534
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Cycle

Description

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Antibody Name:Anti-CDC25B Antibody
Antibody SKU:CAB0534
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human CDC25B (NP_068659.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:1000
Reactivity:Human
Positive Samples:A-549
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human CDC25B (NP_068659.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MEVP QPEP APGS ALSP AGVC GGAQ RPGH LPGL LLGS HGLL GSPV RAAA SSPV TTLT QTMH DLAG LGSE TPKS QVGT LLFR SRSR LTHL SLSR RASE SSLS SESS ESSD AGLC MDSP SPMD PHMA EQTF EQAI QAAS RIIR NEQF AIRR FQSM PVRL LGHS PVLR NITN SQAP DGRR KSEA GSGA ASSS GEDK ENDG FVFK MPWK PTHP SSTH ALAE WASR REAF AQRP SSAP DLMC LSPD RKME VEEL SP
Gene ID:994
Uniprot:P30305
Cellular Location:Cytoplasm, centrosome, cytoskeleton, microtubule organizing center, spindle pole
Calculated MW:60kDa/63kDa/64kDa/67kDa
Observed MW:62kDa
Synonyms:CDC25B
Background:CDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist.
UniProt Protein Function:CDC25B: a member of the MPI phosphatase family. Activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. Shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. At least four transcript variants for this gene exist. Three splice variant isoforms have been described.
UniProt Protein Details:

Protein type:EC 3.1.3.48; Nuclear receptor co-regulator; Protein phosphatase, dual-specificity; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 20p13

Cellular Component: nucleoplasm; spindle pole; centrosome; cytoplasm; cytosol

Molecular Function:protein binding; protein tyrosine phosphatase activity; protein kinase binding

Biological Process: positive regulation of cytokinesis; mitosis; cell division; positive regulation of protein kinase activity; positive regulation of cell proliferation; mitotic cell cycle; G2/M transition of mitotic cell cycle; positive regulation of mitotic cell cycle; protein amino acid phosphorylation; oocyte maturation; female meiosis I

NCBI Summary:CDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist. [provided by RefSeq, Jul 2008]
UniProt Code:P30305
NCBI GenInfo Identifier:4757950
NCBI Gene ID:994
NCBI Accession:NP_004349.1
UniProt Secondary Accession:P30305,O43551, Q13971, Q5JX77, Q6RSS1, Q9BRA6, D3DVY1 D3DVY2, D3DVY3, D3DVY4,
UniProt Related Accession:P30305
Molecular Weight:64,987 Da
NCBI Full Name:M-phase inducer phosphatase 2 isoform 2
NCBI Synonym Full Names:cell division cycle 25B
NCBI Official Symbol:CDC25B  
NCBI Protein Information:M-phase inducer phosphatase 2; dual specificity phosphatase Cdc25B
UniProt Protein Name:M-phase inducer phosphatase 2
UniProt Synonym Protein Names:Dual specificity phosphatase Cdc25B
UniProt Gene Name:CDC25B  
UniProt Entry Name:MPIP2_HUMAN
Anti-CDC25B Antibody (CAB0534)Western blot analysis of extracts of A-549 cells, using CDC25B antibody (CAB0534) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
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