KO Validated Antibodies 2

Anti-CDA Antibody (CAB19845)[KO Validated]

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SKU:
CAB19845
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-CDA Antibody [KO Validated]
Antibody SKU:CAB19845
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant protein of human CDA.
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100
Reactivity:Human, Mouse
Positive Samples:HeLa
Immunogen:Recombinant protein of human CDA.
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:978
Uniprot:P32320
Cellular Location:
Calculated MW:16kDa
Observed MW:16KDa
Anti-CDA Antibody [KO Validated] (CAB19845)Western blot analysis of extracts from normal (control) and CDA knockout (KO) HeLa cells, using CDA antibody (CAB19845) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
Synonyms:CDA, CDD
Background:This gene encodes an enzyme involved in pyrimidine salvaging. The encoded protein forms a homotetramer that catalyzes the irreversible hydrolytic deamination of cytidine and deoxycytidine to uridine and deoxyuridine, respectively. It is one of several deaminases responsible for maintaining the cellular pyrimidine pool. Mutations in this gene are associated with decreased sensitivity to the cytosine nucleoside analogue cytosine arabinoside used in the treatment of certain childhood leukemias.
UniProt Protein Function:CDA: This enzyme scavenge exogenous and endogenous cytidine and 2'-deoxycytidine for UMP synthesis. Belongs to the cytidine and deoxycytidylate deaminase family.
UniProt Protein Details:

Protein type:Nucleotide Metabolism - pyrimidine; Hydrolase; Xenobiotic Metabolism - drug metabolism - other enzymes; EC 3.5.4.5

Chromosomal Location of Human Ortholog: 1p36.2-p35

Cellular Component: extracellular region; cytosol

Molecular Function:protein homodimerization activity; zinc ion binding; nucleoside binding; cytidine deaminase activity

Biological Process: cell surface receptor linked signal transduction; pyrimidine base metabolic process; cytosine metabolic process; nucleobase, nucleoside and nucleotide metabolic process; pyrimidine nucleoside salvage; cytidine deamination; negative regulation of cell growth; pyrimidine salvage; negative regulation of nucleotide metabolic process; protein homotetramerization

NCBI Summary:This gene encodes an enzyme involved in pyrimidine salvaging. The encoded protein forms a homotetramer that catalyzes the irreversible hydrolytic deamination of cytidine and deoxycytidine to uridine and deoxyuridine, respectively. It is one of several deaminases responsible for maintaining the cellular pyrimidine pool. Mutations in this gene are associated with decreased sensitivity to the cytosine nucleoside analogue cytosine arabinoside used in the treatment of certain childhood leukemias. [provided by RefSeq, Jul 2008]
UniProt Code:P32320
NCBI GenInfo Identifier:1705718
NCBI Gene ID:978
NCBI Accession:P32320.2
UniProt Related Accession:P32320
Molecular Weight:
NCBI Full Name:Cytidine deaminase
NCBI Synonym Full Names:cytidine deaminase
NCBI Official Symbol:CDA  
NCBI Official Synonym Symbols:CDD  
NCBI Protein Information:cytidine deaminase
UniProt Protein Name:Cytidine deaminase
UniProt Synonym Protein Names:Cytidine aminohydrolase
Protein Family:Colicin
UniProt Gene Name:CDA  
UniProt Entry Name:CDD_HUMAN
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