Immunology Antibodies 1

Anti-CD8A Antibody (CAB11856)

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SKU:
CAB11856
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology

Description

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Antibody Name:Anti-CD8A Antibody
Antibody SKU:CAB11856
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 22-182 of human CD8A (NP_001759.3).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:Mouse pancreas
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 22-182 of human CD8A (NP_001759.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:SQFR VSPL DRTW NLGE TVEL KCQV LLSN PTSG CSWL FQPR GAAA SPTF LLYL SQNK PKAA EGLD TQRF SGKR LGDT FVLT LSDF RREN EGYY FCSA LSNS IMYF SHFV PVFL PAKP TTTP APRP PTPA PTIA SQPL SLRP EACR PAAG GAVH TRGL DFAC D
Gene ID:925
Uniprot:P01732
Cellular Location:Cell membrane, Secreted, Single-pass type I membrane protein
Calculated MW:21kDa/25kDa/30kDa
Observed MW:30kDa
Synonyms:CD8A, CD8, Leu2, MAL, p32
Background:The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain. Multiple transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:Function: Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
UniProt Protein Details:

Subunit structure: In general heterodimer of an alpha and a beta chain linked by two disulfide bonds. Can also form homodimers. Shown to be expressed as heterodimer on thymocytes and as homodimer on peripheral blood T-lymphocytes. Interacts with the MHC class I HLA-A/B2M dimer. Interacts with LCK in a zinc-dependent manner. Ref.9

Subcellular location: Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted.

Post-translational modification: All of the five most C-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not

By similarity.

Involvement in Disease: CD8 deficiency, familial (CD8 deficiency) [MIM:608957]: An immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.Note: The disease is caused by mutations affecting the gene represented in this entry.

Sequence similarities: Contains 1 Ig-like V-type (immunoglobulin-like) domain.

NCBI Summary:The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]
UniProt Code:P01732
NCBI GenInfo Identifier:116035
NCBI Gene ID:925
NCBI Accession:P01732.1
UniProt Secondary Accession:P01732,Q13970, Q4ZG17, D6W5M8,
UniProt Related Accession:P01732
Molecular Weight:
NCBI Full Name:T-cell surface glycoprotein CD8 alpha chain
NCBI Synonym Full Names:CD8a molecule
NCBI Official Symbol:CD8A  
NCBI Official Synonym Symbols:CD8; MAL; p32; Leu2  
NCBI Protein Information:T-cell surface glycoprotein CD8 alpha chain; T8 T-cell antigen; T cell co-receptor; OKT8 T-cell antigen; T-cell antigen Leu2; Leu2 T-lymphocyte antigen; CD8 antigen, alpha polypeptide (p32); T-lymphocyte differentiation antigen T8/Leu-2
UniProt Protein Name:T-cell surface glycoprotein CD8 alpha chain
UniProt Synonym Protein Names:T-lymphocyte differentiation antigen T8/Leu-2
Protein Family:CD81 antigen
UniProt Gene Name:CD8A  
UniProt Entry Name:CD8A_HUMAN
Anti-CD8A Antibody (CAB11856)Western blot analysis of extracts of mouse pancreas, using CD8A antibody (CAB11856) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-CD8A Antibody (CAB11856)Immunohistochemistry of paraffin-embedded mouse spleen using CD8A Rabbit pAb (CAB11856) at dilution of 1:100 (40x lens).
Anti-CD8A Antibody (CAB11856)Immunohistochemistry of paraffin-embedded rat spleen using CD8A Rabbit pAb (CAB11856) at dilution of 1:100 (40x lens).
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