Antibody Name:	Anti-CD82 Antibody
Antibody SKU:	CAB1778
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 200 to the C-terminus of human CD82 (NP_002222.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	MCF7

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 200 to the C-terminus of human CD82 (NP_002222.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	TQSG NHPE DWPV YQEG CMEK VQAW LQEN LGII LGVG VGVA IIEL LGMV LSIC LCRH VHSE DYSK VPKY
Gene ID:	3732
Uniprot:	P27701
Cellular Location:	Membrane, Multi-pass membrane protein
Calculated MW:	26kDa/29kDa
Observed MW:	45KDa

Synonyms:

CD82, 4F9, C33, GR15, IA4, KAI1, R2, SAR2, ST6, TSPAN27

Background:

This metastasis suppressor gene product is a membrane glycoprotein that is a member of the transmembrane 4 superfamily. Expression of this gene has been shown to be downregulated in tumor progression of human cancers and can be activated by p53 through a consensus binding sequence in the promoter. Its expression and that of p53 are strongly correlated, and the loss of expression of these two proteins is associated with poor survival for prostate cancer patients. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

UniProt Protein Function:	CD82: a widespread transmembrane protein of the tetraspanin family. A metastasis-suppressor whose decreased expression may be involved in malignant progression. Suppresses tumor metastasis of many cancers including cancers of the prostate, bladder, colon, cervix, liver, and lung (NSCLC). It is a target of estrogen receptor-mediated gene repression and is downregulated in primary human breast cancer. May be a prognostic marker for lung cancer and tumor metastatic potential. Interacts with cell surface proteins including integrins, cadherins, CD4, CD8, IGSF8. Modulates EGFR signaling. May suppress invasion by inhibiting integrin-dependent crosstalk with c-Met receptor and Src kinases. Its regulation of c-Met signaling apparently affects cancer cell migration.
UniProt Protein Details:	Protein type:Cell surface; Membrane protein, multi-pass; Membrane protein, integral Chromosomal Location of Human Ortholog: 11p11.2 Cellular Component: integral to plasma membrane; plasma membrane Molecular Function:protein binding Disease: Prostate Cancer
NCBI Summary:	This metastasis suppressor gene product is a membrane glycoprotein that is a member of the transmembrane 4 superfamily. Expression of this gene has been shown to be downregulated in tumor progression of human cancers and can be activated by p53 through a consensus binding sequence in the promoter. Its expression and that of p53 are strongly correlated, and the loss of expression of these two proteins is associated with poor survival for prostate cancer patients. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	P27701
NCBI GenInfo Identifier:	4504813
NCBI Gene ID:	3732
NCBI Accession:	NP_002222
UniProt Secondary Accession:	P27701,Q7Z2D4, Q7Z5N2, D3DQN6, E9PC70,
UniProt Related Accession:	P27701
Molecular Weight:	26,818 Da
NCBI Full Name:	CD82 antigen isoform 1
NCBI Synonym Full Names:	CD82 molecule
NCBI Official Symbol:	CD82
NCBI Official Synonym Symbols:	R2; 4F9; C33; IA4; ST6; GR15; KAI1; SAR2; TSPAN27
NCBI Protein Information:	CD82 antigen; tspan-27; C33 antigen; tetraspanin-27; inducible membrane protein R2; metastasis suppressor Kangai-1; kangai 1 (suppression of tumorigenicity 6, prostate; CD82 antigen (R2 leukocyte antigen, antigen detected by monoclonal and antibody IA4))
UniProt Protein Name:	CD82 antigen
UniProt Synonym Protein Names:	C33 antigen; IA4; Inducible membrane protein R2; Metastasis suppressor Kangai-1; Suppressor of tumorigenicity 6 protein; Tetraspanin-27; Tspan-27; CD_antigen: CD82
Protein Family:	CD82 antigen
UniProt Gene Name:	CD82
UniProt Entry Name:	CD82_HUMAN

	Western blot analysis of extracts of MCF7 cells, using CD82 antibody (CAB1778) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human colon using CD82 antibody (CAB1778) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human spleen using CD82 antibody (CAB1778) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse brain using CD82 antibody (CAB1778) at dilution of 1:100 (40x lens).