Immunology Antibodies 2
Anti-CD74 Antibody (CAB5667)
- SKU:
- CAB5667
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Immunology
Description
| Antibody Name: | Anti-CD74 Antibody |
| Antibody SKU: | CAB5667 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 73-232 of human CD74 (NP_004346.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Rat |
| Positive Samples: | THP-1, HL-60 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 73-232 of human CD74 (NP_004346.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | QQQG RLDK LTVT SQNL QLEN LRMK LPKP PKPV SKMR MATP LLMQ ALPM GALP QGPM QNAT KYGN MTED HVMH LLQN ADPL KVYP PLKG SFPE NLRH LKNT METI DWKV FESW MHHW LLFE MSRH SLEQ KPTD APPK ESLE LEDP SSGL GVTK QDLG PVPM |
| Gene ID: | 972 |
| Uniprot: | P04233 |
| Cellular Location: | Cell membrane, Endoplasmic reticulum membrane, Endosome, Golgi apparatus, Lysosome, Single-pass type II membrane protein, trans-Golgi network |
| Calculated MW: | 18kDa/26kDa/33kDa |
| Observed MW: | 34kDa |
| Synonyms: | CD74, DHLAG, HLADG, II, Ia-GAMMA |
| Background: | The protein encoded by this gene associates with class II major histocompatibility complex (MHC) and is an important chaperone that regulates antigen presentation for immune response. It also serves as cell surface receptor for the cytokine macrophage migration inhibitory factor (MIF) which, when bound to the encoded protein, initiates survival pathways and cell proliferation. This protein also interacts with amyloid precursor protein (APP) and suppresses the production of amyloid beta (Abeta). Multiple alternatively spliced transcript variants encoding different isoforms have been identified. |
| UniProt Protein Function: | Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal/lysosomal system where the antigen processing and binding of antigenic peptides to MHC class II takes place. Serves as cell surface receptor for the cytokine MIF. |
| NCBI Summary: | The protein encoded by this gene associates with class II major histocompatibility complex (MHC) and is an important chaperone that regulates antigen presentation for immune response. It also serves as cell surface receptor for the cytokine macrophage migration inhibitory factor (MIF) which, when bound to the encoded protein, initiates survival pathways and cell proliferation. This protein also interacts with amyloid precursor protein (APP) and suppresses the production of amyloid beta (Abeta). Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011] |
| UniProt Code: | P04233 |
| NCBI GenInfo Identifier: | 20178292 |
| NCBI Gene ID: | 972 |
| NCBI Accession: | P04233.3 |
| UniProt Secondary Accession: | P04233,Q14597, Q29832, Q5U0J8, Q8SNA0, Q8WLP6, A8K7R1 B4DNE8, D3DQG3, D3DQG4, |
| UniProt Related Accession: | P04233 |
| Molecular Weight: | |
| NCBI Full Name: | HLA class II histocompatibility antigen gamma chain |
| NCBI Synonym Full Names: | CD74 molecule |
| NCBI Official Symbol: | CD74 |
| NCBI Official Synonym Symbols: | II; DHLAG; HLADG; Ia-GAMMA |
| NCBI Protein Information: | HLA class II histocompatibility antigen gamma chain |
| UniProt Protein Name: | HLA class II histocompatibility antigen gamma chain |
| UniProt Synonym Protein Names: | HLA-DR antigens-associated invariant chain; Ia antigen-associated invariant chain; Ii; p33; CD_antigen: CD74 |
| Protein Family: | H-2 class II histocompatibility antigen |
| UniProt Gene Name: | CD74 |
| UniProt Entry Name: | HG2A_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CD74 antibody (CAB5667) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
 | Immunofluorescence analysis of C6 cells using CD74 antibody (CAB5667) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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