Antibody Name:	Anti-CD70 Antibody
Antibody SKU:	CAB2032
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 39-193 of human CD70 (NP_001243.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HL-60, Mouse liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 39-193 of human CD70 (NP_001243.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	QRFA QAQQ QLPL ESLG WDVA ELQL NHTG PQQD PRLY WQGG PALG RSFL HGPE LDKG QLRI HRDG IYMV HIQV TLAI CSST TASR HHPT TLAV GICS PASR SISL LRLS FHQG CTIA SQRL TPLA RGDT LCTN LTGT LLPS RNTD ETFF GVQW VRP
Gene ID:	970
Uniprot:	P32970
Cellular Location:	Membrane, Single-pass type II membrane protein
Calculated MW:	21kDa/23kDa
Observed MW:	23kDa

Synonyms:

CD70, CD27-L, CD27L, CD27LG, TNFSF7, TNLG8A

Background:

The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for TNFRSF27/CD27. It is a surface antigen on activated, but not on resting, T and B lymphocytes. It induces proliferation of costimulated T cells, enhances the generation of cytolytic T cells, and contributes to T cell activation. This cytokine is also reported to play a role in regulating B-cell activation, cytotoxic function of natural killer cells, and immunoglobulin sythesis.

UniProt Protein Function:	CD70: Cytokine that binds to CD27. Plays a role in T-cell activation. Induces the proliferation of costimulated T-cells and enhances the generation of cytolytic T-cells. Belongs to the tumor necrosis factor family.
UniProt Protein Details:	Protein type:Membrane protein, integral; Cytokine Chromosomal Location of Human Ortholog: 19p13 Cellular Component: extracellular space; integral to plasma membrane; plasma membrane Molecular Function:protein binding; protease binding; cytokine activity; tumor necrosis factor receptor binding; receptor binding Biological Process: cell proliferation; cell-cell signaling; immune response; signal transduction
NCBI Summary:	The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for TNFRSF27/CD27. It is a surface antigen on activated, but not on resting, T and B lymphocytes. It induces proliferation of costimulated T cells, enhances the generation of cytolytic T cells, and contributes to T cell activation. This cytokine is also reported to play a role in regulating B-cell activation, cytotoxic function of natural killer cells, and immunoglobulin sythesis. [provided by RefSeq, Jul 2008]
UniProt Code:	P32970
NCBI GenInfo Identifier:	21431837
NCBI Gene ID:	970
NCBI Accession:	P32970.2
UniProt Secondary Accession:	P32970,Q53XX4, Q96J57, B4DPR8,
UniProt Related Accession:	P32970
Molecular Weight:	193
NCBI Full Name:	CD70 antigen
NCBI Synonym Full Names:	CD70 molecule
NCBI Official Symbol:	CD70
NCBI Official Synonym Symbols:	CD27L; CD27LG; TNFSF7
NCBI Protein Information:	CD70 antigen; CD27-L; CD27 ligand; Ki-24 antigen; surface antigen CD70; tumor necrosis factor ligand superfamily member 7; tumor necrosis factor (ligand) superfamily, member 7
UniProt Protein Name:	CD70 antigen
UniProt Synonym Protein Names:	CD27 ligand; CD27-L; Tumor necrosis factor ligand superfamily member 7; CD_antigen: CD70
Protein Family:	CD70 antigen
UniProt Gene Name:	CD70
UniProt Entry Name:	CD70_HUMAN

	Western blot analysis of extracts of various cell lines, using CD70 antibody (CAB2032) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunohistochemistry of paraffin-embedded human spleen using CD70 Rabbit pAb (CAB2032) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of Jurkat cells using CD70 Rabbit pAb (CAB2032) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.