Antibody Name:	Anti-CD59 Antibody
Antibody SKU:	CAB1689
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant protein of human CD59

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	SW480, HeLa, SKOV3, Mouse liver, Mouse thymus, Mouse spleen, Rat kidney

Immunogen:	Recombinant protein of human CD59
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	966
Uniprot:	P13987
Cellular Location:	Cell membrane, GPI-anchor, Lipid-anchor, Secreted
Calculated MW:	14kDa
Observed MW:	17kDa

Synonyms:

CD59, 16.3A5, 1F5, EJ16, EJ30, EL32, G344, HRF-20, HRF20, MAC-IP, MACIF, MEM43, MIC11, MIN1, MIN2, MIN3, MIRL, MSK21, p18-20

Background:

This gene encodes a cell surface glycoprotein that regulates complement-mediated cell lysis, and it is involved in lymphocyte signal transduction. This protein is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. This protein also plays a role in signal transduction pathways in the activation of T cells. Mutations in this gene cause CD59 deficiency, a disease resulting in hemolytic anemia and thrombosis, and which causes cerebral infarction. Multiple alternatively spliced transcript variants, which encode the same protein, have been identified for this gene.

UniProt Protein Function:	CD59: Potent inhibitor of the complement membrane attack complex (MAC) action. Acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. Involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase. Defects in CD59 are the cause of CD59 deficiency (CD59D).
UniProt Protein Details:	Protein type:Membrane protein, GPI anchor Chromosomal Location of Human Ortholog: 11p13 Cellular Component: anchored to external side of plasma membrane; cell surface; endoplasmic reticulum membrane; ER-Golgi intermediate compartment membrane; extracellular space; focal adhesion; Golgi membrane; membrane; plasma membrane; vesicle Molecular Function:complement binding; protein binding Biological Process: blood coagulation; cell activation; cell surface receptor linked signal transduction; COPII coating of Golgi vesicle; ER to Golgi vesicle-mediated transport; neutrophil degranulation; regulation of complement activation Disease: Hemolytic Anemia, Cd59-mediated, With Or Without Immune-mediated Polyneuropathy
NCBI Summary:	This gene encodes a cell surface glycoprotein that regulates complement-mediated cell lysis, and it is involved in lymphocyte signal transduction. This protein is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. This protein also plays a role in signal transduction pathways in the activation of T cells. Mutations in this gene cause CD59 deficiency, a disease resulting in hemolytic anemia and thrombosis, and which causes cerebral infarction. Multiple alternatively spliced transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	P13987
NCBI GenInfo Identifier:	116021
NCBI Gene ID:	966
NCBI Accession:	P13987.1
UniProt Related Accession:	P13987
Molecular Weight:	14kDa
NCBI Full Name:	CD59 glycoprotein
NCBI Synonym Full Names:	CD59 molecule (CD59 blood group)
NCBI Official Symbol:	CD59
NCBI Official Synonym Symbols:	1F5; EJ16; EJ30; EL32; G344; MIN1; MIN2; MIN3; MIRL; HRF20; MACIF; MEM43; MIC11; MSK21; 16.3A5; HRF-20; MAC-IP; p18-20
NCBI Protein Information:	CD59 glycoprotein
UniProt Protein Name:	CD59 glycoprotein
UniProt Synonym Protein Names:	1F5 antigen; 20 kDa homologous restriction factor; HRF-20; HRF20; MAC-inhibitory protein; MAC-IP; MEM43 antigen; Membrane attack complex inhibition factor; MACIF; Membrane inhibitor of reactive lysis; MIRL; Protectin; CD_antigen: CD59
Protein Family:	CD59 glycoprotein
UniProt Gene Name:	CD59

	Western blot analysis of extracts of various cell lines, using CD59 antibody (CAB1689) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 60s.
	Immunohistochemistry of paraffin-embedded mouse lung using CD59 antibody (CAB1689) (40x lens).
	Immunohistochemistry of paraffin-embedded rat heart using CD59 antibody (CAB1689) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human lung cancer using CD59 antibody (CAB1689) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
Anti-CD59 Antibody (CAB12412)	Human CD59 ELISA Kit
Anti-CD59 Antibody (CAB4090)	Human CD59 (Protectin) CLIA Kit (HUES00444)
CD59 Antibody (PACO01958)	Human CD59 (Protectin) ELISA Kit (HUES01836)
CD59 Antibody (PACO08298)	Human CD59 glycoprotein (CD59) ELISA Kit