Immunology Antibodies 1

Anti-CD55 Antibody (CAB1228)

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SKU:
CAB1228
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology

Description

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Antibody Name:Anti-CD55 Antibody
Antibody SKU:CAB1228
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 40-300 of human CD55 (NP_000565.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse
Positive Samples:HeLa, A-549, DU145, MCF7, 293T
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 40-300 of human CD55 (NP_000565.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:PDVP NAQP ALEG RTSF PEDT VITY KCEE SFVK IPGE KDSV ICLK GSQW SDIE EFCN RSCE VPTR LNSA SLKQ PYIT QNYF PVGT VVEY ECRP GYRR EPSL SPKL TCLQ NLKW STAV EFCK KKSC PNPG EIRN GQID VPGG ILFG ATIS FSCN TGYK LFGS TSSF CLIS GSSV QWSD PLPE CREI YCPA PPQI DNGI IQGE RDHY GYRQ SVTY ACNK GFTM IGEH SIYC TVNN DEGE WSGP PPEC RGKS LTSK VPPT VQKP T
Gene ID:1604
Uniprot:P08174
Cellular Location:Cell membrane, GPI-anchor, Lipid-anchor, Secreted, Single-pass type I membrane protein
Calculated MW:39kDa/41kDa/48kDa/56kDa/59kDa
Observed MW:55kDa
Synonyms:CD55, CR, CROM, DAF, TC
Background:This gene encodes a glycoprotein involved in the regulation of the complement cascade. Binding of the encoded protein to complement proteins accelerates their decay, thereby disrupting the cascade and preventing damage to host cells. Antigens present on this protein constitute the Cromer blood group system (CROM). Alternative splicing results in multiple transcript variants. The predominant transcript variant encodes a membrane-bound protein, but alternatively spliced transcripts may produce soluble proteins.
UniProt Protein Function:CD55: This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade. Belongs to the receptors of complement activation (RCA) family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Membrane protein, GPI anchor; Cell surface

Chromosomal Location of Human Ortholog: 1q32

Cellular Component: cell surface; integral to plasma membrane; extracellular region; plasma membrane; lipid raft

Molecular Function:viral receptor activity; protein binding; lipid binding

Biological Process: respiratory burst; entry of virus into host cell; elevation of cytosolic calcium ion concentration; negative regulation of complement activation; regulation of complement activation; innate immune response; regulation of lipopolysaccharide-mediated signaling pathway; complement activation, classical pathway

Disease: Blood Group, Cromer System

NCBI Summary:This gene encodes a protein involved in the regulation of the complement cascade. The encoded glycoprotein is also known as the decay-accelerating factor (DAF); binding of DAF to complement proteins accelerates their decay, disrupting the cascade and preventing damage to host cells. Antigens present on the DAF glycoprotein constitute the Cromer blood group system (CROM). Two alternatively spliced transcripts encoding different proteins have been identified. The predominant transcript encodes a membrane-bound protein expressed on cells exposed to plasma component proteins but an alternatively spliced transcript produces a soluble protein present at much lower levels. Additional, alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]
UniProt Code:P08174
NCBI GenInfo Identifier:10835143
NCBI Gene ID:1604
NCBI Accession:NP_000565.1
UniProt Secondary Accession:P08174,P09679, P78361, B1AP14, D3DT83, D3DT84,
UniProt Related Accession:P08174
Molecular Weight:41,400 Da
NCBI Full Name:complement decay-accelerating factor isoform 1 preproprotein
NCBI Synonym Full Names:CD55 molecule, decay accelerating factor for complement (Cromer blood group)
NCBI Official Symbol:CD55  
NCBI Official Synonym Symbols:CR; TC; DAF; CROM  
NCBI Protein Information:complement decay-accelerating factor; CD55 antigen
UniProt Protein Name:Complement decay-accelerating factor
Protein Family:Complement decay-accelerating factor
UniProt Gene Name:CD55  
UniProt Entry Name:DAF_HUMAN
Anti-CD55 Antibody (CAB1228)Western blot analysis of extracts of various cell lines, using CD55 antibody (CAB1228) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Anti-CD55 Antibody (CAB1228)Immunofluorescence analysis of A549 cells using CD55 antibody (CAB1228). Blue: DAPI for nuclear staining.
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