Cell Biology Antibodies 11

Anti-CD47 Antibody (CAB7278)

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SKU:
CAB7278
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-CD47 Antibody
Antibody SKU:CAB7278
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 20-140 of human CD47 (NP_942088.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:Mouse brain, mouse lung, rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 20-140 of human CD47 (NP_942088.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:LLFN KTKS VEFT FCND TVVI PCFV TNME AQNT TEVY VKWK FKGR DIYT FDGA LNKS TVPT DFSS AKIE VSQL LKGD ASLK MDKS DAVS HTGN YTCE VTEL TREG ETII ELKY RVVS WFSP N
Gene ID:961
Uniprot:Q08722
Cellular Location:Cell membrane, Multi-pass membrane protein
Calculated MW:31kDa/33kDa/35kDa
Observed MW:47kDa
Synonyms:CD47, IAP, MER6, OA3
Background:This gene encodes a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This gene has broad tissue distribution, and is reduced in expression on Rh erythrocytes. Alternatively spliced transcript variants have been found for this gene.
UniProt Protein Function:CD47: Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus. Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Membrane protein, integral; Membrane protein, multi-pass; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 3q13.1-q13.2

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function:protein binding

Biological Process: integrin-mediated signaling pathway; extracellular matrix organization and biogenesis; response to bacterium; positive regulation of phagocytosis; positive regulation of cell proliferation; positive regulation of cell-cell adhesion; positive regulation of T cell activation; opsonization; blood coagulation; cell adhesion; leukocyte migration; positive regulation of inflammatory response

NCBI Summary:This gene encodes a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This gene has broad tissue distribution, and is reduced in expression on Rh erythrocytes. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2010]
UniProt Code:Q08722
NCBI GenInfo Identifier:1171879
NCBI Gene ID:961
NCBI Accession:Q08722.1
UniProt Related Accession:Q08722
Molecular Weight:
NCBI Full Name:Leukocyte surface antigen CD47
NCBI Synonym Full Names:CD47 molecule
NCBI Official Symbol:CD47  
NCBI Official Synonym Symbols:IAP; OA3; MER6  
NCBI Protein Information:leukocyte surface antigen CD47
UniProt Protein Name:Leukocyte surface antigen CD47
UniProt Synonym Protein Names:Antigenic surface determinant protein OA3; Integrin-associated protein; IAP; Protein MER6; CD_antigen: CD47
Protein Family:IgE-binding protein
UniProt Gene Name:CD47  
UniProt Entry Name:CD47_HUMAN
Anti-CD47 Antibody (CAB7278)Western blot analysis of extracts of mouse brain, using CD47 Antibody (CAB7278) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-CD47 Antibody (CAB7278)Western blot analysis of extracts of various cell lines, using CD47 Antibody (CAB7278) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
Anti-CD47 Antibody (CAB7278)Immunofluorescence analysis of Jurkat cells using CD47 antibody (CAB7278) at dilution of 1:100. Blue: DAPI for nuclear staining.
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