Description
| Antibody Name: | Anti-CD46 Antibody |
| Antibody SKU: | CAB13515 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 40-320 of human CD46 (NP_722548.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human |
| Positive Samples: | HT-29 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 40-320 of human CD46 (NP_722548.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | TFEA MELI GKPK PYYE IGER VDYK CKKG YFYI PPLA THTI CDRN HTWL PVSD DACY RETC PYIR DPLN GQAV PANG TYEF GYQM HFIC NEGY YLIG EEIL YCEL KGSV AIWS GKPP ICEK VLCT PPPK IKNG KHTF SEVE VFEY LDAV TYSC DPAP GPDP FSLI GEST IYCG DNSV WSRA APEC KVVK CRFP VVEN GKQI SGFG KKFY YKAT VMFE CDKG FYLD GSDT IVCD SNST WDPP VPKC LKVS TSST TKSP ASSA SGPR PTYK PPVS NYPG YPKP E |
| Gene ID: | 4179 |
| Uniprot: | P15529 |
| Cellular Location: | Cytoplasmic vesicle, Single-pass type I membrane protein, acrosome inner membrane, secretory vesicle |
| Calculated MW: | 36kDa/39kDa/40kDa/41kDa/42kDa/43kDa/44kDa |
| Observed MW: | 44kDa |
| Synonyms: | AHUS2, MCP, MIC10, TLX, TRA2.10, CD46 |
| Background: | The protein encoded by this gene is a type I membrane protein and is a regulatory part of the complement system. The encoded protein has cofactor activity for inactivation of complement components C3b and C4b by serum factor I, which protects the host cell from damage by complement. In addition, the encoded protein can act as a receptor for the Edmonston strain of measles virus, human herpesvirus-6, and type IV pili of pathogenic Neisseria. Finally, the protein encoded by this gene may be involved in the fusion of the spermatozoa with the oocyte during fertilization. Mutations at this locus have been associated with susceptibility to hemolytic uremic syndrome. Alternatively spliced transcript variants encoding different isoforms have been described. |
| UniProt Protein Function: | CD46: Acts as a cofactor for complement factor I, a serine protease which protects autologous cells against complement- mediated injury by cleaving C3b and C4b deposited on host tissue. May be involved in the fusion of the spermatozoa with the oocyte during fertilization. Also acts as a costimulatory factor for T- cells which induces the differentiation of CD4+ into T-regulatory 1 cells. T-regulatory 1 cells suppress immune responses by secreting interleukin-10, and therefore are thought to prevent autoimmunity. A number of viral and bacterial pathogens seem to exploit this property and directly induce an immunosuppressive phenotype in T-cells by binding to CD46. Defects in CD46 are a cause of susceptibility to hemolytic uremic syndrome atypical type 2 (AHUS2). An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype. Patients with CD46 mutations seem to have an overall better prognosis compared to patients carrying CFH mutations. 16 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Membrane protein, integral; Receptor, misc.; Cell surface Chromosomal Location of Human Ortholog: 1q32 Cellular Component: Golgi apparatus; inner acrosomal membrane; focal adhesion; cell surface; basolateral plasma membrane; integral to plasma membrane; plasma membrane Molecular Function:enzyme inhibitor activity; complement binding; protein binding; cadherin binding; endopeptidase activity; receptor activity Biological Process: positive regulation of memory T cell differentiation; adaptive immune response; positive regulation of regulatory T cell differentiation; viral reproduction; T cell mediated immunity; proteolysis; positive regulation of interleukin-10 production; regulation of Notch signaling pathway; negative regulation of complement activation; single fertilization; negative regulation of catalytic activity; interleukin-10 production; regulation of complement activation; innate immune response; positive regulation of T cell proliferation; complement activation, classical pathway |
| NCBI Summary: | The protein encoded by this gene is a type I membrane protein and is a regulatory part of the complement system. The encoded protein has cofactor activity for inactivation of complement components C3b and C4b by serum factor I, which protects the host cell from damage by complement. In addition, the encoded protein can act as a receptor for the Edmonston strain of measles virus, human herpesvirus-6, and type IV pili of pathogenic Neisseria. Finally, the protein encoded by this gene may be involved in the fusion of the spermatozoa with the oocyte during fertilization. Mutations at this locus have been associated with susceptibility to hemolytic uremic syndrome. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jun 2010] |
| UniProt Code: | P15529 |
| NCBI GenInfo Identifier: | 41019474 |
| NCBI Gene ID: | 4179 |
| NCBI Accession: | P15529.3 |
| UniProt Secondary Accession: | P15529,Q15429, Q53GV9, Q5HY94, Q5VWS6, Q5VWS7, Q5VWS8 Q5VWS9, Q5VWT0, A0T1T0, A0T1T1, A0T1T2, |
| UniProt Related Accession: | P15529 |
| Molecular Weight: | 392 |
| NCBI Full Name: | Membrane cofactor protein |
| NCBI Synonym Full Names: | CD46 molecule, complement regulatory protein |
| NCBI Official Symbol: | CD46Â Â |
| NCBI Official Synonym Symbols: | MCP; TLX; AHUS2; MIC10; TRA2.10Â Â |
| NCBI Protein Information: | membrane cofactor protein; measles virus receptor; complement membrane cofactor protein; trophoblast leucocyte common antigen; trophoblast leukocyte common antigen; CD46 antigen, complement regulatory protein; trophoblast-lymphocyte cross-reactive antigen |
| UniProt Protein Name: | Membrane cofactor protein |
| UniProt Synonym Protein Names: | TLX; Trophoblast leukocyte common antigen; CD_antigen: CD46 |
| Protein Family: | Membrane cofactor protein |
| UniProt Gene Name: | CD46Â Â |
| UniProt Entry Name: | MCP_HUMAN |
 | Western blot analysis of extracts of HT-29 cells, using CD46 antibody (CAB13515) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
 | Immunofluorescence analysis of HepG2 cells using CD46 antibody (CAB13515). Blue: DAPI for nuclear staining. |
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