Immunology Antibodies 1
Anti-CD3D Antibody (CAB1238)
- SKU:
- CAB1238
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Immunology
Description
| Antibody Name: | Anti-CD3D Antibody |
| Antibody SKU: | CAB1238 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 22-105 of human CD3D (NP_000723.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100 |
| Reactivity: | Human, Rat |
| Positive Samples: | Jurkat |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 22-105 of human CD3D (NP_000723.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | FKIP IEEL EDRV FVNC NTSI TWVE GTVG TLLS DITR LDLG KRIL DPRG IYRC NGTD IYKD KEST VQVH YRMC QSCV ELDP ATVA |
| Gene ID: | 915 |
| Uniprot: | P04234 |
| Cellular Location: | Membrane, Single-pass type I membrane protein |
| Calculated MW: | 14kDa/18kDa |
| Observed MW: | 19kDa |
| Synonyms: | CD3D, CD3-DELTA, IMD19, T3D |
| Background: | The protein encoded by this gene is part of the T-cell receptor/CD3 complex (TCR/CD3 complex) and is involved in T-cell development and signal transduction. The encoded membrane protein represents the delta subunit of the CD3 complex, and along with four other CD3 subunits, binds either TCR alpha/beta or TCR gamma/delta to form the TCR/CD3 complex on the surface of T-cells. Defects in this gene are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (SCIDBNK). Two transcript variants encoding different isoforms have been found for this gene. Other variants may also exist, but the full-length natures of their transcripts has yet to be defined. |
| UniProt Protein Function: | CD3D: a T cell surface glycoprotein that is a component of the T cell antigen receptor. A defect in this protein causes a severe combined immunodeficiency characterized by the absence of T cells but normal B cells. Contains 1 ITAM domain. |
| UniProt Protein Details: | Protein type:Membrane protein, integral; Receptor, misc. Chromosomal Location of Human Ortholog: 11q23 Cellular Component: T cell receptor complex; cytoplasm; plasma membrane; integral to membrane; alpha-beta T cell receptor complex Molecular Function:transmembrane receptor activity; protein heterodimerization activity; transcription coactivator activity Biological Process: regulation of immune response; cell surface receptor linked signal transduction; T cell costimulation; positive regulation of transcription from RNA polymerase II promoter; T cell receptor signaling pathway; T cell differentiation; positive thymic T cell selection Disease: Immunodeficiency 19 |
| NCBI Summary: | The protein encoded by this gene is part of the T-cell receptor/CD3 complex (TCR/CD3 complex) and is involved in T-cell development and signal transduction. The encoded membrane protein represents the delta subunit of the CD3 complex, and along with four other CD3 subunits, binds either TCR alpha/beta or TCR gamma/delta to form the TCR/CD3 complex on the surface of T-cells. Defects in this gene are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (SCIDBNK). Two transcript variants encoding different isoforms have been found for this gene. Other variants may also exist, but the full-length natures of their transcripts has yet to be defined. [provided by RefSeq, Feb 2009] |
| UniProt Code: | P04234 |
| NCBI GenInfo Identifier: | 115985 |
| NCBI Gene ID: | 915 |
| NCBI Accession: | P04234.1 |
| UniProt Secondary Accession: | P04234,A8MVP6, |
| UniProt Related Accession: | P04234 |
| Molecular Weight: | 171 |
| NCBI Full Name: | T-cell surface glycoprotein CD3 delta chain |
| NCBI Synonym Full Names: | CD3d molecule, delta (CD3-TCR complex) |
| NCBI Official Symbol: | CD3D |
| NCBI Official Synonym Symbols: | T3D; IMD19; CD3-DELTA |
| NCBI Protein Information: | T-cell surface glycoprotein CD3 delta chain; OKT3, delta chain; CD3 antigen, delta subunit; T-cell receptor T3 delta chain; CD3d antigen, delta polypeptide (TiT3 complex) |
| UniProt Protein Name: | T-cell surface glycoprotein CD3 delta chain |
| UniProt Synonym Protein Names: | T-cell receptor T3 delta chain; CD_antigen: CD3d |
| Protein Family: | CD302 antigen |
| UniProt Gene Name: | CD3D |
| UniProt Entry Name: | CD3D_HUMAN |
 | Western blot analysis of extracts of Jurkat cells, using CD3D antibody (CAB1238) at 1:500 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded rat spleen using CD3D antibody (CAB1238) at dilution of 1:200 (40x lens). |
 | Immunofluorescence analysis of Jurkat cells using CD3D antibody (CAB1238) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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