KO Validated Antibodies 1

Anti-CD34 Antibody (CAB7429)[KO Validated]

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SKU:
CAB7429
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-CD34 Antibody [KO Validated]
Antibody SKU:CAB7429
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 160-320 of human CD34 (NP_001020280).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:293T, Mouse heart, Mouse pancreas
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 160-320 of human CD34 (NP_001020280).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:PYTS SSPI LSDI KAEI KCSG IREV KLTQ GICL EQNK TSSC AEFK KDRG EGLA RVLC GEEQ ADAD AGAQ VCSL LLAQ SEVR PQCL LLVL ANRT EISS KLQL MKKH QSDL KKLG ILDF TEQD VASH QSYS QKTL IALV TSGA LLAV LGIT GYFL MNRR SWSP T
Gene ID:947
Uniprot:P28906
Cellular Location:Membrane, Single-pass type I membrane protein
Calculated MW:35kDa/40kDa
Observed MW:110-120kDa
Synonyms:CD34, CD34 molecule, GIG3, MORT1
Background:The protein encoded by this gene may play a role in the attachment of stem cells to the bone marrow extracellular matrix or to stromal cells. This single-pass membrane protein is highly glycosylated and phosphorylated by protein kinase C. Two transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:CD34: Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins. Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues. Belongs to the CD34 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Cell surface; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q32

Cellular Component: intercellular bridge; integral to plasma membrane; lysosome; perinuclear region of cytoplasm; apical plasma membrane; cytoplasm; basal plasma membrane; plasma membrane; external side of plasma membrane

Molecular Function:sulfate binding; carbohydrate binding; transcription factor binding

Biological Process: positive regulation of odontogenesis; regulation of immune response; negative regulation of nitric oxide biosynthetic process; glomerular filtration; cell motility involved in cell locomotion; negative regulation of blood coagulation; negative regulation of tumor necrosis factor production; signal transduction; positive regulation of interleukin-10 production; cell proliferation; cell-cell adhesion; positive regulation of angiogenesis; endothelial cell proliferation; regulation of blood pressure; tissue homeostasis; hemopoiesis; leukocyte migration

NCBI Summary:The protein encoded by this gene may play a role in the attachment of stem cells to the bone marrow extracellular matrix or to stromal cells. This single-pass membrane protein is highly glycosylated and phosphorylated by protein kinase C. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]
UniProt Code:P28906
NCBI GenInfo Identifier:3183511
NCBI Gene ID:947
NCBI Accession:P28906.2
UniProt Secondary Accession:P28906,Q15970, Q15971, Q5JTA3, Q5JTA4, Q9UJB1, A8K664
UniProt Related Accession:P28906
Molecular Weight:35,011 Da
NCBI Full Name:Hematopoietic progenitor cell antigen CD34
NCBI Synonym Full Names:CD34 molecule
NCBI Official Symbol:CD34  
NCBI Protein Information:hematopoietic progenitor cell antigen CD34; CD34 antigen
UniProt Protein Name:Hematopoietic progenitor cell antigen CD34
UniProt Synonym Protein Names:CD_antigen: CD34
Protein Family:Hematopoietic progenitor cell antigen
UniProt Gene Name:CD34  
UniProt Entry Name:CD34_HUMAN
Anti-CD34 Antibody [KO Validated] (CAB7429)Western blot analysis of extracts of various cell lines, using CD34 antibody (CAB7429) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-CD34 Antibody [KO Validated] (CAB7429)Western blot analysis of extracts from normal (control) and CD34 Rabbit pAb knockout (KO) 293T cells, using CD34 Rabbit pAb antibody (CAB7429) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-CD34 Antibody [KO Validated] (CAB7429)Immunofluorescence analysis of TF-1 cells using CD34 antibody (CAB7429) at dilution of 1:300. Blue: DAPI for nuclear staining.
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