Immunology Antibodies 1
Anti-CD300LF Antibody (CAB13830)
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- SKU:
- CAB13830
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Immunology
Description
| Antibody Name: | Anti-CD300LF Antibody |
| Antibody SKU: | CAB13830 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 23-150 of human CD300LF (NP_001276011.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human |
| Positive Samples: | NCI-H460, K-562, U-937 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 23-150 of human CD300LF (NP_001276011.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | TQIT GPTT VNGL ERGS LTVQ CVYR SGWE TYLK WWCR GAIW RDCK ILVK TSGS EQEV KRDR VSIK DNQK NRTF TVTM EDLM KTDA DTYW CGIE KTGN DLGV TVQV TIDP APVT QEET SSSP TLTG HHLD |
| Gene ID: | 146722 |
| Uniprot: | Q8TDQ1 |
| Cellular Location: | Cell membrane, Single-pass type I membrane protein |
| Calculated MW: | 18kDa/21kDa/26kDa/32kDa |
| Observed MW: | 36kDa |
| Synonyms: | CD300LF, CD300f, CLM-1, CLM1, IREM-1, IREM1, IgSF13, LMIR3, NKIR |
| Background: | This gene encodes a member of the CD300 protein family. Members of this family are cell surface glycoproteins with a single IgV-like extracellular domain, and are involved in the regulation of immune response. The encoded protein is an inhibitory receptor. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | IREM-1: Acts as an inhibitory receptor for myeloid cells and mast cells. Inhibits osteoclast formation. Belongs to the CD300 family. 6 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Immunoglobulin superfamily; Receptor, misc.; Membrane protein, integral Chromosomal Location of Human Ortholog: 17q25.1 Cellular Component: integral to membrane; plasma membrane Biological Process: immune system process; regulation of immune response |
| NCBI Summary: | This gene encodes a member of the CD300 protein family. Members of this family are cell surface glycoproteins with a single IgV-like extracellular domain, and are involved in the regulation of immune response. The encoded protein is an inhibitory receptor. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014] |
| UniProt Code: | Q8TDQ1 |
| NCBI GenInfo Identifier: | 296439398 |
| NCBI Gene ID: | 146722 |
| NCBI Accession: | Q8TDQ1.3 |
| UniProt Secondary Accession: | Q8TDQ1,Q3Y6P0, Q6UX24, Q7Z6A6, Q7Z7I4, Q7Z7I5, Q8N6D0 Q8NAF5, B2RCL2, C9JDN3, |
| UniProt Related Accession: | Q8TDQ1 |
| Molecular Weight: | 32,628 Da |
| NCBI Full Name: | CMRF35-like molecule 1 |
| NCBI Synonym Full Names: | CD300 molecule like family member f |
| NCBI Official Symbol: | CD300LF |
| NCBI Official Synonym Symbols: | CLM1; NKIR; CLM-1; IREM1; LMIR3; CD300f; IREM-1; IgSF13 |
| NCBI Protein Information: | CMRF35-like molecule 1 |
| UniProt Protein Name: | CMRF35-like molecule 1 |
| UniProt Synonym Protein Names: | CD300 antigen-like family member F; Immune receptor expressed on myeloid cells 1; IREM-1; Immunoglobulin superfamily member 13; IgSF13; NK inhibitory receptor; CD_antigen: CD300f |
| Protein Family: | CMRF35-like molecule |
| UniProt Gene Name: | CD300LF |
| UniProt Entry Name: | CLM1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CD300LF antibody (CAB13830) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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