Cell Death Antibodies 1

Anti-CD27 Antibody (CAB1945)

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SKU:
CAB1945
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Death

Description

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Antibody Name:Anti-CD27 Antibody
Antibody SKU:CAB1945
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 20-191 of human CD27 (NP_001233.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:Raji, HeLa, Jurkat, HL-60, Mouse thymus
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 20-191 of human CD27 (NP_001233.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:ATPA PKSC PERH YWAQ GKLC CQMC EPGT FLVK DCDQ HRKA AQCD PCIP GVSF SPDH HTRP HCES CRHC NSGL LVRN CTIT ANAE CACR NGWQ CRDK ECTE CDPL PNPS LTAR SSQA LSPH PQPT HLPY VSEM LEAR TAGH MQTL ADFR QLPA RTLS THWP PQRS LCSS DFIR
Gene ID:939
Uniprot:P26842
Cellular Location:Membrane, Single-pass type I membrane protein
Calculated MW:29kDa
Observed MW:50-55kDa
Synonyms:CD27, S152, S152. LPFS2, T14, TNFRSF7, Tp55, S152.LPFS2
Background:The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor.
UniProt Protein Function:CD27: Receptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1.
UniProt Protein Details:

Protein type:Membrane protein, integral; Apoptosis; Receptor, cytokine

Chromosomal Location of Human Ortholog: 12p13

Cellular Component: integral to plasma membrane; neuron projection; nucleus; plasma membrane

Molecular Function:caspase inhibitor activity; protein binding; tumor necrosis factor receptor activity

Biological Process: brain development; immune response; induction of apoptosis via death domain receptors; inflammatory response; negative regulation of apoptosis; positive regulation of JNK cascade; positive regulation of MAPKKK cascade; regulation of cell proliferation; response to lipopolysaccharide; tumor necrosis factor-mediated signaling pathway

Disease: Lymphoproliferative Syndrome 2

NCBI Summary:The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor. [provided by RefSeq, Jul 2008]
UniProt Code:P26842
NCBI GenInfo Identifier:269849546
NCBI Gene ID:939
NCBI Accession:P26842.2
UniProt Secondary Accession:P26842,B2RDZ0,
UniProt Related Accession:P26842
Molecular Weight:29,137 Da
NCBI Full Name:CD27 antigen
NCBI Synonym Full Names:CD27 molecule
NCBI Official Symbol:CD27  
NCBI Official Synonym Symbols:T14; S152; Tp55; TNFRSF7; S152. LPFS2  
NCBI Protein Information:CD27 antigen
UniProt Protein Name:CD27 antigen
UniProt Synonym Protein Names:CD27L receptor; T-cell activation antigen CD27; T14; Tumor necrosis factor receptor superfamily member 7; CD_antigen: CD27
Protein Family:CD27 antigen
UniProt Gene Name:CD27  
UniProt Entry Name:CD27_HUMAN
Anti-CD27 Antibody (CAB1945)Western blot analysis of extracts of various cell lines, using CD27 antibody (CAB1945) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-CD27 Antibody (CAB1945)Immunohistochemistry of paraffin-embedded rat spleen using CD27 antibody (CAB1945) at dilution of 1:100 (40x lens).
Anti-CD27 Antibody (CAB1945)Immunohistochemistry of paraffin-embedded mouse spleen using CD27 antibody (CAB1945) at dilution of 1:100 (40x lens).
Anti-CD27 Antibody (CAB1945)Immunofluorescence analysis of Jurkat cells using CD27 antibody (CAB1945) at dilution of 1:100. Blue: DAPI for nuclear staining.
Anti-CD27 Antibody (CAB1945)Immunofluorescence analysis of rat thymus using CD27 antibody (CAB1945) at dilution of 1:100. Blue: DAPI for nuclear staining.
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